Lab July 27 2009

From 2009.igem.org

(Difference between revisions)
(New page: Checked plates - growth on all 4 plates new DH5alpha plates look contaminated. Staph? Not sure. old competent cells look OK. Should we just go back to our old cell line? It seems t...)
 
Line 28: Line 28:
after mixing each plasmid concentration, follow the Transformation Protocol.
after mixing each plasmid concentration, follow the Transformation Protocol.
 +
 +
 +
 +
 +
Back to [https://2009.igem.org/Team:VictoriaBC/Labnotebook LAB NOTEBOOK]

Latest revision as of 22:26, 17 October 2009

Checked plates - growth on all 4 plates


new DH5alpha plates look contaminated. Staph? Not sure.


old competent cells look OK. Should we just go back to our old cell line? It seems the issue was a plasmid dilution issue rather than a competent cells issue.


re-plating with the following plasmid dilutions:


currently have 23ng/ul, to get to 10ng/ul mix 4.35ul plasmid and 5.65 ul dH2O


1 ng = 1ul of previous mix in 9ul of dH2O


.01 ng = 1ul of previous mix in 9ul of dH2O


after mixing each plasmid concentration, follow the Transformation Protocol.



Back to LAB NOTEBOOK