Lab July 27 2009
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Jasoncummer (Talk | contribs) (New page: Checked plates - growth on all 4 plates new DH5alpha plates look contaminated. Staph? Not sure. old competent cells look OK. Should we just go back to our old cell line? It seems t...) |
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after mixing each plasmid concentration, follow the Transformation Protocol. | after mixing each plasmid concentration, follow the Transformation Protocol. | ||
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+ | Back to [https://2009.igem.org/Team:VictoriaBC/Labnotebook LAB NOTEBOOK] |
Latest revision as of 22:26, 17 October 2009
Checked plates - growth on all 4 plates
new DH5alpha plates look contaminated. Staph? Not sure.
old competent cells look OK. Should we just go back to our old cell line? It seems the issue was a plasmid dilution issue rather than a competent cells issue.
re-plating with the following plasmid dilutions:
currently have 23ng/ul, to get to 10ng/ul mix 4.35ul plasmid and 5.65 ul dH2O
1 ng = 1ul of previous mix in 9ul of dH2O
.01 ng = 1ul of previous mix in 9ul of dH2O
after mixing each plasmid concentration, follow the Transformation Protocol.
Back to LAB NOTEBOOK