Team:Brown/Notebook Recipes/Ampicillin
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(New page: {{Brown}} Making Ampicillin (Amp) Stock = 100x (Meaning use 1ul of Amp Stock for Every 1ml of Medium) → [50ug/ml] ---- • Making 10 of 1ml aliquots 1. 0.5g of Amp (Prevent it from ...) |
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Latest revision as of 07:03, 20 October 2009
Making Ampicillin (Amp) Stock = 100x (Meaning use 1ul of Amp Stock for Every 1ml of Medium) → [50ug/ml]
• Making 10 of 1ml aliquots 1. 0.5g of Amp (Prevent it from light exposure) 2. 10ml ddH2O ➢ Mix the solution until the Amp is completely dissolved (It SHOULD NOT sink to the bottom of the tube because the solution must be homogenous) ➢ Aliquot into 1ml microcentrifuge tubes and store in -20oC.