Team:Valencia/Notebook/July

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| 13 ||[[Team:Valencia/Notebook/July#July 14th|14]] || [[Team:Valencia/Notebook/July#July 15th|15]] || &nbsp;[[Team:Valencia/Notebook/July#July 16th|16]] || [[Team:Valencia/Notebook/July#July 17th|17]] || [[Team:Valencia/Notebook/July#July 18th|18]] || 19 ||  
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| 27||[[Team:Valencia/Notebook/July#July 28th|28]]|| [[Team:Valencia/Notebook/July#July 29th|29]] || &nbsp;[[Team:Valencia/Notebook/July#July 30th|30]] || [[Team:Valencia/Notebook/July#July 31st|31]] || <Font Color="#c0c0c0">&nbsp;&nbsp;1</Font> || <Font Color="#c0c0c0">&nbsp;2</Font>
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=== July 1st ===
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=== July 28th ===
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- We prepared some [[Team:Valencia/Notebook/Protocols#Glycerol Stock | glycerol stock]] from the four ''Saccharomyces cerevisiae'' strains we received from [http://www.cbm.uam.es/mitolab/fichapersonal.aspx?idpersona=6 Eduardo Rial]  : <br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Negative control, '''UCP -'''. <br>
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Today we have prepared the SD medium lacking Leu that we need for the yeast experiment. We have 500 ml  of solid medium and 500 ml of liquid medium. That should be more than enough for all the summer.
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Strain containing UCP1, '''UCP +'''. <br>
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The components of the medium are:
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Mutant stains over expressing UCP1, '''Gly 76Δ''' and '''Gly 175Δ'''. <br>
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 +
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- 0.5% (NH4)2SO4
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- 0.17% Yeast Synthetic Drop-Out Medium Supplement Without Leucine
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- We calibrated our calorimeters with hot water.
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- 2% Glucose
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=== July 2nd ===
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- 1'5% Agar (only for the solid medium)
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- We prepared our first [[Team:Valencia/Notebook/Protocols#SD Medium | SD medium.]]
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=== July 3rd ===
 
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- We work at lab
 
<br><br><br><br><br><br>
<br><br><br><br><br><br>

Latest revision as of 07:22, 20 October 2009




April
Mo Tu We Th Fr Sa Su
30 31  1  2  3  4  5
 6  7  8  9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30  1  2  3
May
Mo Tu We Th Fr Sa Su
27 28 29 30  1  2  3
 4  5  6  7  8  9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
Mo Tu We Th Fr Sa Su
 1  2  3  4  5  6  7
 8  9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30  1   2  3  4  5
July
Mo Tu We Th Fr Sa Su
29 30  1  2  3  4  5
 6  7  8  9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31   1  2
August
Mo Tu We Th Fr Sa Su
27 28 29  30 31  1  2
 3  4  5  6  7  8  9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31 1 2 3 4 5 6
September
Mo Tu We Th Fr Sa Su
31  1  2  3  4  5  6
 7  8  9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30  1 2 3 4
October
Mo Tu We Th Fr Sa Su
28 29 30   1  2  3  4
 5  6  7  8  9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31  1
November
Mo Tu We Th Fr Sa Su
26 27 28  29 30 31  1
 2  3  4  5  6  7  8
 9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30  1  2  3  4  5  6

July 28th

Today we have prepared the SD medium lacking Leu that we need for the yeast experiment. We have 500 ml of solid medium and 500 ml of liquid medium. That should be more than enough for all the summer. The components of the medium are:


- 0.5% (NH4)2SO4

- 0.17% Yeast Synthetic Drop-Out Medium Supplement Without Leucine

- 2% Glucose

- 1'5% Agar (only for the solid medium)








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