Team:UNIPV-Pavia/Notebook/Week3Dec

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__NOTOC__
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<html><a name="week_start"></a></html>
= Week from December 15th, to December 21st, 2008 =
= Week from December 15th, to December 21st, 2008 =
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Mar#week_start">Next Week</a>
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Mar#week_start">
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== <font class="dayw_style">December, 16th</font> ==
== <font class="dayw_style">December, 16th</font> ==
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Official presentation of the project "Engineering E. coli to multiplex and demultiplex signals" by UNIPV-Pavia iGEM 2008 team to the main academic authorities:
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*Rector of 'Università Degli Studi di Pavia', Prof. Angiolino Stella
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*Engineering Dean of 'Università Degli Studi di Pavia', Prof. Carlo Ciaponi
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*Director of Department of Biomedical Engineering, Prog. Giovanni Magenes
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During the conference, the president of 'Fondazione Costa' awarded Lorenzo and Mattia, 2008 iGEM students, for the excellent results achieved in the previous competition.
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All the studentes interested in took part in the 2009 iGEM team were present.
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[[Image:UNIPV_presentazione.png| 650px |thumb|center|Award Ceremony]]
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===Trasformation of Ligations===
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A5: BBa_Q0040 with BBa_I732017 (RBS-TETR-PTET-RBS-LACZ-TT)<br>
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A6: BBa_J23100 with BBa_Q0040 (PCON-RBS-TETR-TT-PTET )
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* 1 ul A5 in Top10 Competent
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* 1 ul A6 in Top10 Competent
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Mar#week_start">Next Week</a>
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Mar#week_start">
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Mix 80 ul of SOC with 20 ul X-GAL (40 mg/ml) and infect LB+Amp Agar plates.
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<img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
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* Add 250 ul SOC in 50 ul A5+ Top10
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* Add 250 ul SOC in 50 ul A6+ Top10
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1 hr incubation at 37°C (falcons with shaking 220 rpm) <br>
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Infect:
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# X-GAL plate with 200 ul A5
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# X-GAL plate with 100 ul A5
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# Agar plate with 200 ul A6
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# X-GAL plate with 20 ul of BBa_E0240 from glycerol stock (negative control).
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Expected results:
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*Plates 1 and 2 should develop blue colonies
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*Plate 4 should remain colorless
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16 hr incubation at 37°C.
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Latest revision as of 08:54, 20 October 2009

EthanolPVanimation.gif

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November 2009
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Week from December 15th, to December 21st, 2008

Next Week

December, 16th

Official presentation of the project "Engineering E. coli to multiplex and demultiplex signals" by UNIPV-Pavia iGEM 2008 team to the main academic authorities:

  • Rector of 'Università Degli Studi di Pavia', Prof. Angiolino Stella
  • Engineering Dean of 'Università Degli Studi di Pavia', Prof. Carlo Ciaponi
  • Director of Department of Biomedical Engineering, Prog. Giovanni Magenes

During the conference, the president of 'Fondazione Costa' awarded Lorenzo and Mattia, 2008 iGEM students, for the excellent results achieved in the previous competition. All the studentes interested in took part in the 2009 iGEM team were present.


Award Ceremony

Top

Next Week

Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Dec"