"
Team:EPF-Lausanne/Results/Mutations
From 2009.igem.org
(Difference between revisions)
| Line 15: | Line 15: | ||
</html> | </html> | ||
| + | |||
| + | <html><center> | ||
| + | <font size="10" color="#007CBC">Mutations</font> | ||
| + | </center></html> | ||
| + | <br><br> | ||
| + | Starting from the result of the differential analysis, we decided to mutate some residues around CYS450. | ||
| + | |||
| + | |||
| + | =Active site= | ||
| + | The selected residues for mutations are ILE427 in purple on the next video, LEU453 in orange and VAL416 in yellow. | ||
| + | <html><center><object width="425" height="344"><param name="movie" value="http://www.youtube.com/v/m-eLb7OV00Q&hl=fr&fs=1&"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/m-eLb7OV00Q&hl=fr&fs=1&" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="425" height="344"></embed></object></center></html> | ||
| + | |||
| + | =ILE427 to PHE= | ||
| + | |||
| + | =LEU453 to GLY= | ||
| + | |||
| + | =VAL416 to ILE= | ||
| + | |||
| + | <br> | ||
<html> | <html> | ||
<br><center> | <br><center> | ||
| Line 36: | Line 55: | ||
</html> | </html> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
</div><div CLASS="epfl09bouchon"></div> | </div><div CLASS="epfl09bouchon"></div> | ||
Revision as of 09:48, 20 October 2009
Starting from the result of the differential analysis, we decided to mutate some residues around CYS450.
Active site
The selected residues for mutations are ILE427 in purple on the next video, LEU453 in orange and VAL416 in yellow.
ILE427 to PHE
LEU453 to GLY
VAL416 to ILE
