Team:KU Seoul/Result

From 2009.igem.org

(Difference between revisions)
(→Results)
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:1. Synthetic Circuit - I : pSB3C5/Pars-gfp-Pznt-rfp
:1. Synthetic Circuit - I : pSB3C5/Pars-gfp-Pznt-rfp
::1)Pznt-rfp
::1)Pznt-rfp
 +
 +
:This parts is producing red fluorosence based on the zinc ion level. The zinc detecting promoter can be
 +
:induced beyond 1mM concentration of zinc ion. This range from 1mM to 2mM is proper to detect zinc level on
 +
:the common waste water. Maybe it is required to detect the upper concentration of zinc ion in waste water that
 +
:using the dilution method which dilute the level of zinc concentration. If the level curve is too rugh or not
 +
:too much change according to concentraion, you need to apply dilution or concentration of sample.
 +
{| style="color:Black; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1"
{| style="color:Black; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1"
|align="center"|Zn<Sup>2+</Sup> (mM)  
|align="center"|Zn<Sup>2+</Sup> (mM)  
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::2)Pars-gfp
::2)Pars-gfp
-
This parts is producing red fluorosence based on the zinc ion level. The zinc detecting promoter can be induced beyond 1mM concentration of zinc ion. This range from 1mM to 2mM is proper to detect zinc level on the common waste water. Maybe it is required to detect the upper concentration of zinc ion in waste water that using the dilution method which dilute the level of zinc concentration. If the level curve is too rugh or not too much change according to concentraion, you need to apply dilution or concentration of sample.
+
 
{| style="color:Black; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1"
{| style="color:Black; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1"

Revision as of 13:55, 20 October 2009






Results

1. Synthetic Circuit - I : pSB3C5/Pars-gfp-Pznt-rfp
1)Pznt-rfp
This parts is producing red fluorosence based on the zinc ion level. The zinc detecting promoter can be
induced beyond 1mM concentration of zinc ion. This range from 1mM to 2mM is proper to detect zinc level on
the common waste water. Maybe it is required to detect the upper concentration of zinc ion in waste water that
using the dilution method which dilute the level of zinc concentration. If the level curve is too rugh or not
too much change according to concentraion, you need to apply dilution or concentration of sample.
Zn2+ (mM) 0 0.5 0.75 1 1.5 2
OD600 0.490 ± 0.003 0.459 ± 0.004 0.433 ± 0.008 0.418 ± 0.005 0.275 ± 0.011 0.243 ± 0.008
Red fluorescence (RF) 26628 ± 706 27856 ± 292 29333 ± 292 31217 ± 66 46044 ± 237 49188 ± 174
OD600/RF 54376 ± 1203 60646 ± 787 67803 ± 573 74687 ± 755 167833 ± 7202 202565 ± 6428
Background 0 6270 ± 520 13427 ± 1719 20311 ± 475 113457 ± 6016 148189 ± 6731

KU Seoul Result RFP.png

2)Pars-gfp


AsO3- (mM) 0 0.00625 0.125 0.25 0.5 0.75 1
OD600 0.553 ± 0.009 0.519 ± 0.002 0.480 ± 0.005 0.395 ± 0.007 0.315 ± 0.006 0.286 ± 0.004 0.273 ± 0.006
Green fluorescence (GF) 27740 ± 226 26411 ± 169 25267 ± 267 25536 ± 213 25407 ± 259 24849 ± 152 26479 ± 149
OD600/GF 50206 ± 1225 50855 ± 123 52606 ± 689 64669 ± 1684 80665 ± 603 86897 ± 1321 96784 ± 2165
Background 0 648 ± 1314 2399 ± 969 14463 ± 2896 30459 ± 1631 36691 ± 1636 46578 ± 3220

KU Seoul Result GFP.png KU Seoul Calibrationcurve.png

2.Synthetic circuit – II : PyodA-AMD
Cd2+ (mM) 0 0.05 0.1 0.2 0.4 0.8
OD615 0.242 ± 0.005 0.258 ± 0.008 0.274 ± 0.011 0.284 ± 0.01 0.293 ± 0.011 0.302 ± 0.01
Background 0 0.016 ± 0.004 0.032 ± 0.006 0.043 ± 0.005 0.052 ± 0.007 0.060 ± 0.007

KU Seoul Expression.png

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