From 2009.igem.org
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| [[Image:KU Seoul Result RFP.png]] | | [[Image:KU Seoul Result RFP.png]] |
| ::2)Pars-gfp | | ::2)Pars-gfp |
- | | + | :Differing from zinc ion, Arsenic ion is more severe impact on the E.coli. When microbials are exposed by |
| + | :arsenic ion even 1mM concentration, This concentraion of arsenic ion is already full of toxicity. It is very |
| + | :senstive to detect arsenic ion. It means that arsenic ion can provide strong harmful effect on the organism : |
| + | :even microbial which is known as the best survivor on the earth. The interaction of arsenic ion is participated |
| + | :in more than two or more group of proteins or others. See the curve of fluorosence of GFP. |
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Revision as of 14:03, 20 October 2009
Results
- 1. Synthetic Circuit - I : pSB3C5/Pars-gfp-Pznt-rfp
- 1)Pznt-rfp
- This parts is producing red fluorosence based on the zinc ion level. The zinc detecting promoter can be
- induced beyond 1mM concentration of zinc ion. This range from 1mM to 2mM is proper to detect zinc level on
- the common waste water. Maybe it is required to detect the upper concentration of zinc ion in waste water that
- using the dilution method which dilute the level of zinc concentration. If the level curve is too rugh or not
- too much change according to concentraion, you need to apply dilution or concentration of sample.
Zn2+ (mM)
| 0
| 0.5
| 0.75
| 1
| 1.5
| 2
|
OD600
| 0.490 ± 0.003
| 0.459 ± 0.004
| 0.433 ± 0.008
| 0.418 ± 0.005
| 0.275 ± 0.011
| 0.243 ± 0.008
|
Red fluorescence (RF)
| 26628 ± 706
| 27856 ± 292
| 29333 ± 292
| 31217 ± 66
| 46044 ± 237
| 49188 ± 174
|
OD600/RF
| 54376 ± 1203
| 60646 ± 787
| 67803 ± 573
| 74687 ± 755
| 167833 ± 7202
| 202565 ± 6428
|
Background
| 0
| 6270 ± 520
| 13427 ± 1719
| 20311 ± 475
| 113457 ± 6016
| 148189 ± 6731
|
- 2)Pars-gfp
- Differing from zinc ion, Arsenic ion is more severe impact on the E.coli. When microbials are exposed by
- arsenic ion even 1mM concentration, This concentraion of arsenic ion is already full of toxicity. It is very
- senstive to detect arsenic ion. It means that arsenic ion can provide strong harmful effect on the organism :
- even microbial which is known as the best survivor on the earth. The interaction of arsenic ion is participated
- in more than two or more group of proteins or others. See the curve of fluorosence of GFP.
AsO3- (mM)
| 0
| 0.00625
| 0.125
| 0.25
| 0.5
| 0.75
| 1
|
OD600
| 0.553 ± 0.009
| 0.519 ± 0.002
| 0.480 ± 0.005
| 0.395 ± 0.007
| 0.315 ± 0.006
| 0.286 ± 0.004
| 0.273 ± 0.006
|
Green fluorescence (GF)
| 27740 ± 226
| 26411 ± 169
| 25267 ± 267
| 25536 ± 213
| 25407 ± 259
| 24849 ± 152
| 26479 ± 149
|
OD600/GF
| 50206 ± 1225
| 50855 ± 123
| 52606 ± 689
| 64669 ± 1684
| 80665 ± 603
| 86897 ± 1321
| 96784 ± 2165
|
Background
| 0
| 648 ± 1314
| 2399 ± 969
| 14463 ± 2896
| 30459 ± 1631
| 36691 ± 1636
| 46578 ± 3220
|
- 2.Synthetic circuit – II : PyodA-AMD
Cd2+ (mM)
| 0
| 0.05
| 0.1
| 0.2
| 0.4
| 0.8
|
OD615
| 0.242 ± 0.005
| 0.258 ± 0.008
| 0.274 ± 0.011
| 0.284 ± 0.01
| 0.293 ± 0.011
| 0.302 ± 0.01
|
Background
| 0
| 0.016 ± 0.004
| 0.032 ± 0.006
| 0.043 ± 0.005
| 0.052 ± 0.007
| 0.060 ± 0.007
|
|