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Team:EPF-Lausanne/Protocols/M9medium
From 2009.igem.org
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| - | '''M9 minimal + AA :''' | + | '''M9 minimal + AA + thiamine:''' |
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Latest revision as of 18:46, 20 October 2009
M9 minimal + AA + thiamine:
- 10 ml of M9 salts 10x
- 0.2 ml of MgSO4 (1M)
- 0.1 ml of CaCl2 (0.1 mM)
- 10 ml of glucose (20%) -> we actually now think it's better to put 4% so 20 ml
- 200 mg of AA mix
- 800 ul of uracile
- 800 ul of histidine
- 800 ul of leucine
M9 salts 10x ( -> 500 ml)
- 30 g Na2HPO4 * 7H2O
- 15 g KH2PO4
- 2.5 g NaCl
- 5 g NH4Cl
