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Team:Imperial College London/Wetlab/Results/OD calibration
From 2009.igem.org
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(New page: {{Imperial/09/TemplateTop}} We have calibrated the absorbance at 600nm (O.D.600) with the colony forming units. This allows us to approximate our absorbance data into a cell count number...) |
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We have calibrated the absorbance at 600nm (O.D.600) with the colony forming units. This allows us to approximate our absorbance data into a cell count number. We can now normalise fluorescence data to obtain the fluorescence produced per cell.<br> | We have calibrated the absorbance at 600nm (O.D.600) with the colony forming units. This allows us to approximate our absorbance data into a cell count number. We can now normalise fluorescence data to obtain the fluorescence produced per cell.<br> | ||
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We performed the experiment and further reruns with 2-3 repeats per OD value and for 2 different dilutions.<br> | We performed the experiment and further reruns with 2-3 repeats per OD value and for 2 different dilutions.<br> | ||
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{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Latest revision as of 22:47, 20 October 2009
We have calibrated the absorbance at 600nm (O.D.600) with the colony forming units. This allows us to approximate our absorbance data into a cell count number. We can now normalise fluorescence data to obtain the fluorescence produced per cell.
We performed the experiment and further reruns with 2-3 repeats per OD value and for 2 different dilutions.
