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Team:Warsaw/Sources
From 2009.igem.org
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(New page: {{WarHead1}} =Sources of natural bricks= Below we present sources of bricks prepared by our team. ==Invasiveness bricks== Most of bricks connected with bacteria invasiveness were acquir...) |
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Most of bricks connected with bacteria invasiveness were acquired from following organisms: | Most of bricks connected with bacteria invasiveness were acquired from following organisms: | ||
| - | {|class="wikitable" style="border: 0px; margin: 3px; text-align: justify;" | + | {|class="wikitable" style="border: 0px; margin: 3px; text-align: justify;" width="100%" |
|- | |- | ||
| - | !style="text-align: center"|organism | + | !style="text-align: center" width="45%"|organism |
| - | !style="text-align: center"|genomic sequence details | + | !style="text-align: center" width="30%"|genomic sequence details |
!style="text-align: center"|acquired genes | !style="text-align: center"|acquired genes | ||
|- | |- | ||
| - | |''Salmonella enterolitica'' ser. ''typhimurium'' LT2 | + | |''Salmonella enterolitica'' ser. ''typhimurium'' LT2<sup>1</sup> |
|style="text-align: center;"|[http://www.ncbi.nlm.nih.gov/nuccore/NC_003197 NC_OO3197] | |style="text-align: center;"|[http://www.ncbi.nlm.nih.gov/nuccore/NC_003197 NC_OO3197] | ||
|[https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoP, PhoQ], [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter MgtC promoter] | |[https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoP, PhoQ], [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter MgtC promoter] | ||
|- | |- | ||
| - | |''Listeria monocytogenes | + | |''Listeria monocytogenes'' EGD-E<sup>2</sup> |
| + | |style="text-align: center;"|[http://www.ncbi.nlm.nih.gov/nuccore/NC_003210 NC_003210] | ||
| + | |[https://2009.igem.org/Team:Warsaw/Glossary#listeriolysin listeriolysin], [https://2009.igem.org/Team:Warsaw/Glossary#internalin internalinA] | ||
| + | |- | ||
| + | |''Yersina enterolitica'' O:8 (8081)<sup>3</sup> | ||
| + | |style="text-align: center;"|[http://www.ncbi.nlm.nih.gov/nuccore/AM286415 AM286415] | ||
| + | |[https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | ||
|} | |} | ||
| + | ==Other bricks== | ||
| + | |||
| + | {|class="wikitable" style="border: 0px; margin: 3px; text-align: justify;" width="100%" | ||
| + | |- | ||
| + | !style="text-align: center" width="45%"|name of the brick | ||
| + | !style="text-align: center" width="30%"|sequence details | ||
| + | !style="text-align: center"|source | ||
| + | |- | ||
| + | |style="text-align: center"|CDS of [https://2009.igem.org/Team:Warsaw/Glossary#p53 p53] | ||
| + | |style="text-align: center"|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K177016 BBa_K177016] | ||
| + | |human<sup>4</sup> | ||
| + | |- | ||
| + | |style="text-align: center"|CDS of [https://2009.igem.org/Team:Warsaw/Glossary#bax bax] | ||
| + | |style="text-align: center"|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K177027 BBa_K177027] | ||
| + | |human <sup>5</sup> | ||
| + | |- | ||
| + | |style="text-align: center"|mitochondrial targeting signal from the [https://2009.igem.org/Team:Warsaw/Glossary#cytochrome_C_oxidase Cox] gene | ||
| + | |style="text-align: center"|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K177028 BBa_K177028] | ||
| + | |human<sup>6</sup> | ||
| + | |- | ||
| + | |style="text-align: center"|[https://2009.igem.org/Team:Warsaw/Glossary#Type_I_secretion_system secretion system] | ||
| + | |style="text-align: center"| | ||
| + | |''Escherichia coli''<sup>7</sup> | ||
| + | |} | ||
| + | |||
| + | |||
| + | |||
| + | <sup>1</sup> Genomic DNA from this strain was a generous gift from ... | ||
| + | |||
| + | <sup>2</sup> Genomic DNA from this strain was a generous gift from prof. Jacek Bielecki, PhD, [http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=528&b_2501=5 Laboratory of Applied Microbiology], Faculty of Biology, University of Warsaw | ||
| + | |||
| + | <sup>3</sup> Genomic DNA from this strain was a generous gift from ... | ||
| + | <sup>4</sup> Plasmid carrying p53 gene was a generous gift from Dawid Walerych, PhD, from the [http://www.iimcb.gov.pl/zylicz_lab.php Lab of Molecular Biology], International Institute of Molecular and Cell Biology in Warsaw | ||
| + | <sup>5</sup> Plasmid carrying bax gene was a generous gift from Łukasz Borowski, MSc, from the [http://www.igib.uw.edu.pl/index.php?id=80 Institute of Genetics and Biotechnology], University of Warsaw. | ||
| + | <sup>6</sup> Mitochondrial targeting signal was amplified from the [http://www.clontech.com/images/pt/PT3730-5.pdf pAcGFP-mito] vector | ||
| + | <sup>7</sup> Plasmids carrying essential elements of mentioned secretion system were a generous gift from Carlos A. Guzman; [http://www.helmholtz-hzi.de/en/research/research_groups/vaccinology_and_applied_microbiology/vaccinology/ Department of Vaccinology and Applied Microbiology], Helmholtz Center for Infection Research | ||
{{WarFoot1}} | {{WarFoot1}} | ||
Latest revision as of 23:45, 20 October 2009
Sources of natural bricks
Below we present sources of bricks prepared by our team.
Invasiveness bricks
Most of bricks connected with bacteria invasiveness were acquired from following organisms:
| organism | genomic sequence details | acquired genes |
|---|---|---|
| Salmonella enterolitica ser. typhimurium LT21 | [http://www.ncbi.nlm.nih.gov/nuccore/NC_003197 NC_OO3197] | PhoP, PhoQ, MgtC promoter |
| Listeria monocytogenes EGD-E2 | [http://www.ncbi.nlm.nih.gov/nuccore/NC_003210 NC_003210] | listeriolysin, internalinA |
| Yersina enterolitica O:8 (8081)3 | [http://www.ncbi.nlm.nih.gov/nuccore/AM286415 AM286415] | invasin |
Other bricks
| name of the brick | sequence details | source |
|---|---|---|
| CDS of p53 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177016 BBa_K177016] | human4 |
| CDS of bax | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177027 BBa_K177027] | human 5 |
| mitochondrial targeting signal from the Cox gene | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177028 BBa_K177028] | human6 |
| secretion system | Escherichia coli7 |
1 Genomic DNA from this strain was a generous gift from ...
2 Genomic DNA from this strain was a generous gift from prof. Jacek Bielecki, PhD, [http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=528&b_2501=5 Laboratory of Applied Microbiology], Faculty of Biology, University of Warsaw
3 Genomic DNA from this strain was a generous gift from ...
4 Plasmid carrying p53 gene was a generous gift from Dawid Walerych, PhD, from the [http://www.iimcb.gov.pl/zylicz_lab.php Lab of Molecular Biology], International Institute of Molecular and Cell Biology in Warsaw
5 Plasmid carrying bax gene was a generous gift from Łukasz Borowski, MSc, from the [http://www.igib.uw.edu.pl/index.php?id=80 Institute of Genetics and Biotechnology], University of Warsaw.
6 Mitochondrial targeting signal was amplified from the [http://www.clontech.com/images/pt/PT3730-5.pdf pAcGFP-mito] vector
7 Plasmids carrying essential elements of mentioned secretion system were a generous gift from Carlos A. Guzman; [http://www.helmholtz-hzi.de/en/research/research_groups/vaccinology_and_applied_microbiology/vaccinology/ Department of Vaccinology and Applied Microbiology], Helmholtz Center for Infection Research
