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Team:UNIPV-Pavia/Notebook/Week1Jul
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*We transformed 10 pg of A8 and A9 in TOP10, after a proper quantification with Nanodrop and dilution. We plated transformed bacteria and incubated them overnight at 37°C. | *We transformed 10 pg of A8 and A9 in TOP10, after a proper quantification with Nanodrop and dilution. We plated transformed bacteria and incubated them overnight at 37°C. | ||
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| + | *We prepared 0.5 l of LB + Amp. | ||
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| + | *We infected 5 ml of LB + Amp with 10 ul of T9002 and A10 glycerol stocks. We incubated the cultures overnight at 37°C, 220 rpm. | ||
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''Experiment with Tecan F200'' | ''Experiment with Tecan F200'' | ||
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| + | * <html><u>Description</u></html> | ||
| + | * <html><u>Purpose:</u></html> | ||
| + | * <html><u>Materials & Methods</u></html> | ||
| + | * <html><u>Protocol</u></html> | ||
| + | * <html><u>Results</u></html> | ||
Revision as of 17:58, 1 July 2009
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Week from July 1st, to July 5th, 2009
Previous Week
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Next Week
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July, 1st
- Sequencing results for BOL1 BioBrick: sequence was correct!
- Miniprep for:
- A8 (for dilution/transformation)
- A9 (for dilution/transformation)
- We transformed 10 pg of A8 and A9 in TOP10, after a proper quantification with Nanodrop and dilution. We plated transformed bacteria and incubated them overnight at 37°C.
- We prepared 0.5 l of LB + Amp.
- We infected 5 ml of LB + Amp with 10 ul of T9002 and A10 glycerol stocks. We incubated the cultures overnight at 37°C, 220 rpm.
Preparation of experiment with Tecan F200
- We diluted 1:1000 the cultures of:
- J23100
- J23101
- J23118
- A1
- A2
- A7
- E0240
- We incubated the diluted cultures at 37°C, 220 rpm for 3 hours.
Experiment with Tecan F200
- Description
- Purpose:
- Materials & Methods
- Protocol
- Results
July, 2nd
July, 3rd
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Previous Week
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Next Week
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