Revision as of 18:02, 1 July 2009

Week from July 1st, to July 5th, 2009

Miniprep for:
- A8 (for dilution/transformation)
- A9 (for dilution/transformation)

We transformed 10 pg of A8 and A9 in TOP10, after a proper quantification with Nanodrop and dilution. We plated transformed bacteria and incubated them overnight at 37°C.

We infected 5 ml of LB + Amp with 10 ul of T9002 glycerol stock. We incubated the culture overnight at 37°C, 220 rpm.

We also picked a random colony from A10 plate and infected 1 ml of LB + Amp. We incubated this inoculum at 37°C, 220 rpm for 5 and 1/2 hours. Then we prepared a glycerol stock and re-filled the remaining 250 ul of culture with 5 ml of LB + Amp to grow an overnight culture (for sequencing).

Preparation of experiment with Tecan F200

Experiment with Tecan F200

@@ Line 34: / Line 34: @@
 *We prepared 0.5 l of LB + Amp.
-*We infected 5 ml of LB + Amp with 10 ul of T9002 and A10 glycerol stocks. We incubated the cultures overnight at 37°C, 220 rpm.
+*We infected 5 ml of LB + Amp with 10 ul of T9002 glycerol stock. We incubated the culture overnight at 37°C, 220 rpm.
+*We also picked a random colony from A10 plate and infected 1 ml of LB + Amp. We incubated this inoculum at 37°C, 220 rpm for 5 and 1/2 hours. Then we prepared a glycerol stock and re-filled the remaining 250 ul of culture with 5 ml of LB + Amp to grow an overnight culture (for sequencing).