Week from July 1st, to July 5th, 2009

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July, 1st

• Sequencing results for BOL1 BioBrick: sequence was correct!

• Miniprep for:
  • A8 (for dilution/transformation)
  • A9 (for dilution/transformation)

• We transformed 10 pg of A8 and A9 miniprepped DNA in TOP10, after a proper quantification with Nanodrop and dilution. We plated transformed bacteria and incubated them overnight at 37°C.

• We prepared 0.5 l of LB + Amp.

• We infected 5 ml of LB + Amp with 10 ul of T9002 glycerol stock. We incubated the culture overnight at 37°C, 220 rpm.

• We also picked a random colony from A10 plate and infected 1 ml of LB + Amp. We incubated this inoculum at 37°C, 220 rpm for 5 and 1/2 hours. Then we prepared a glycerol stock and re-filled the remaining 250 ul of culture with 5 ml of LB + Amp to grow an overnight culture (for sequencing).

Preparation of experiment with Tecan F200

• We diluted 1:1000 the cultures of:
  • J23100
  • J23101
  • J23118
  • A1
  • A2
  • A7
  • E0240

• We incubated the diluted cultures at 37°C, 220 rpm for 3 hours.

Experiment with Tecan F200

• Description
• Purpose:
• Materials & Methods
• Protocol
• Results

July, 2nd

• Miniprep for A10 and T9002. We sent purified DNA to BMR Genomics for sequencing.

Preparation of experiment with Tecan F200

• We diluted 1:1000 the cultures of:
  • A8
  • A9
  • E0240

• We incubated the diluted cultures at 37°C, 220 rpm for 3 hours.

Experiment with Tecan F200

• Description
• Purpose:
• Materials & Methods
• Protocol
• Results

July, 3rd

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