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Team:EPF-Lausanne/ODEmodeling
From 2009.igem.org
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| + | The simulation of the expression of the RFP protein activated by the lovTAP in the Read Out 2 circuit was obtained by the following model: | ||
'''1.''' LacI --> LovTAP | '''1.''' LacI --> LovTAP | ||
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* [[Image:delta.png]] is the dilution rate [min -1] | * [[Image:delta.png]] is the dilution rate [min -1] | ||
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| + | The values of these parameters are listed in the table below: | ||
Revision as of 15:19, 21 October 2009
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The simulation of the expression of the RFP protein activated by the lovTAP in the Read Out 2 circuit was obtained by the following model:
1. LacI --> LovTAP
2. LovTAP --> LovTAP* (activated form of LovTAP due to a light-induced conformational change)
3. lovTAP* --> TetR
4. TetR --> RFP
5. RFP --> RFP* (activated, fluorescent)
Where:
- L is the concentration of lovTAP [um]
- L* is the concentration of the activated lovTAP (all lovTAP molecules become activated when exposed to light stimulation) [um]
- T is the concentration of TetR [um]
- R is the concentration of RFP [um]
- R* is the concentration of activated RFP [um]
-
is the function of light exposure
-
is the basal expression of the given promoter
- K is the affinity of the given repressor for its target promoter [um]
- n is the cooperativity coefficient of the Hill function
-
is the molecule degradation rate [min -1]
-
is the dilution rate [min -1]
