Team:Newcastle/Meetings/16 March 2009

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Contents 1 16/03/2009 - iGEM Meeting 1.1 Agenda of the Meeting (16/03/09) 1.2 Agenda of the Meeting (18/03/09) 1.3 NEW Action Points From Last Meeting 1.4 Minutes of the Meeting (16/03/09) 1.5 Action Points of the Meeting (16/03/09) 1.6 Minutes of the Meeting (18/03/09) 1.7 Action Points of the Meeting (16/03/09)

16/03/2009 - iGEM Meeting

Agenda of the Meeting (16/03/09)

• Come up with the project title
• Create the frontpage of the wiki
• Discuss our project title (combination of the 1st and 3rd idea)
• Come up with the paragraph summarising our project idea to be put up on the external wiki

Agenda of the Meeting (18/03/09)

• Introduce ourselves to Nigel Robinson
• Review last meetings action points
• Present our ideas in the form of a flowchart/movie clip
• Feedback and discussion
• Questions
• Show paragraph summarising our idea
• Review additional things

NEW Action Points From Last Meeting

• Review the questions and challenges put forward for each idea
• Do additional reading/research into ideas but more of an emphasis on the first and third ideas
• Finalise an idea and write a paragraph on the idea to be posted in the main wiki
• Reschedule wiki tutorial with Morgan.

Minutes of the Meeting (16/03/09)

• Start: 5p.m at Claremont Tower Room 601, End: 7p.m
• Reviewed previous actions points
  • Funding - Done (Jessica)
• Briefly discussed about BioBricks
• Discussed about the awarding of the medals
  • Our project would probably be based on the environmental side
  • Some of the "easier" awards which can be more "easily" obtained are: Best Wiki, Best Poster, Best Environmental Project, Best Presentations, etc.
  • One of the criteria for the medal is to help a team, helping a new team would probably be easier.
• To make our wiki more attractive, a banner using flash, javascript would be desired, referred to iGEM2008 Heidelberg's wiki as a good reference of a nice wiki.
• Brainstormed on the paragraph on our idea to be put on the external wiki.
• Brainstormed for our title, coming up with keywords such as
  • Heavy metals
  • Stochastic switch
  • Soil/mud
  • Tunable
  • Sporulation
  • Sequester
  • Spores
  • Thousand years memory
  • Scavenge
  • Hunter
  • Seeker
  • Basillus Subtillis
  • Kamikaze/Suicde
  • The Quest to Sequester
• Discussed that, perhaps, with the combination of the two ideas, could just use UV light to shine to "test" the sample, instead of having to take a sample and bringing it back to the lab for testing.
• Discussed that antibodies could be used in identifying the spores.
• Questions
  • How to extract the metals from the cell?
  • How can we get metalloproteins into the cells?
  • Sporulation is induced due to stress, how much metal can we get into the cell?

Action Points of the Meeting (16/03/09)

• Familiarise self with BioBricks, website to go: www.partsregistry.org - ALL
• Craig, Matthew, Arun to come up with the presentation slides, with the addition of pictures, flow diagrams, instead of just words to present the idea.
• Read Nigel Robinson's paper (11 pages) in preparation for Wednesday's (18/03/09) meeting - ALL
• Think of questions to ask regarding Nigel Robinson's paper - ALL

Minutes of the Meeting (18/03/09)

• Craig, Matthew and Arun presented our project idea to the team and guest advisor Nigel Robinson
• Nigel Robinson posted some questions
  • What metals are we interested in?
    • Cadmium, Mercury, Arsenic >> Red List metals
    • Will be difficult to import the red list metals, normally imports Mg, Zn, etc instead and not the red list metals >> Switch the exporter switch for Cd efflux
    • E.Coli has a mercury importer on ...
    • Silver will go in through copper??
    • Importer for Zn in Bacillus, express a protein ...
    • Put Gsh A, Gsh B into ... (from E.Coli)
    • Knock out Cad A, then find the maximum Cad A that survived.

Action Points of the Meeting (16/03/09)

• List all the BioBricks needed
• Develop software systems which stimulates the system
• X-Mind (?)