Team:Newcastle/Modelling/SporulationTuning

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Revision as of 15:54, 21 October 2009

Meetings Calendar

Sporulation Tuning

KinA Expression Model

Under normal conditions, LacI represses KinA.

However, in the presence of IPTG, KinA can be expressed, as IPTG binds to LacI, deactivating it. Equations (a) and (b) describes how IPTG binds to LacI, forming LacI*, which is the deactivated form of LacI

Over time, the deactivated form of LacI, LacI* may degrade. This is also taken into consideration while building the model.

Protein synthesis requires two steps, transcription and translation, and is further illustrated in Figure 1, below.

Figure 1: Transcription and Translation

Referring to Figure 1, the following equations can be written:

Transcription of LacI,

Translation of LacI,

where mRNA_LacI is inducing the formation of LacI.

Transcription of KinA,

where LacI is repressing mRNA_KinA, therefore a lower concentration of LacI would result in a higher concentration of mRNA_KinA.

Translation of KinA,

where mRNA_KinA is inducing the formation of KinA.

20 – 21 June 2009 - Europe workshop (London)
23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
23 October Practice Presentation (Newcastle)
23 October T-shirts are ready
27 October Practice Presentation (Sunderland)
27 October Poster is ready
30 October – 2 November 2009 - Jamboree (Boston)

Newcastle iGEM Twitter
[http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
[http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]

@@ Line 3: / Line 3: @@
 {{:Team:Newcastle/Left}}
 ==Sporulation Tuning==
+===KinA Expression Model===
+Under normal conditions, LacI represses KinA.
+[[Image:TeamNewcastleKinAExpLacIKinA.png|110px|center]]
+However, in the presence of IPTG, KinA can be expressed, as IPTG binds to LacI, deactivating it. Equations (a) and (b) describes how IPTG binds to LacI, forming LacI*, which is the deactivated form of LacI
+[[Image:TeamNewcastleKinAExpLacIIPTG1.png|220px|center]]
+Over time, the deactivated form of LacI, LacI* may degrade. This is also taken into consideration while building the model.
+[[Image:TeamNewcastleKinAExpDeactivatedLacIDegradation.png|80px|center]]
+Protein synthesis requires two steps, transcription and translation, and is further illustrated in Figure 1, below.
+[[Image:TeamNewcastleKinAExpTranscriptiontranslation.png|300px|center]]
+<center>''Figure 1: Transcription and Translation''</center>
+Referring to Figure 1, the following equations can be written:
+;<u>Transcription of LacI,</u>
+[[Image:TeamNewcastleKinAExpLacITranscription.png|120px|center]]
+;<u>Translation of LacI,</u>
+[[Image:TeamNewcastleKinAExpLacITranslation.png|80px|center]]
+where mRNA_LacI is inducing the formation of LacI.
+;<u>Transcription of KinA,</u>
+[[Image:TeamNewcastleKinAExpKinATranscription.png|130px|center]]
+where LacI is repressing mRNA_KinA, therefore a lower concentration of LacI would result in a higher concentration of mRNA_KinA.
+;<u>Translation of KinA,</u>
+[[Image:TeamNewcastleKinAExpKinATranslation.png|80px|center]]
+where mRNA_KinA is inducing the formation of KinA.
 {{:Team:Newcastle/Footer}}
 {{:Team:Newcastle/Right}}