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Uppsala-Sweden/12 October 2009
From 2009.igem.org
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(New page: ==Various== BG-11 plates with kanamycin and without CuSO4 were also made for triparental mating. --Anders 17:09, 11 October 2009 (UTC) ==Transformation== Digestion o...) |
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| + | The colony PCR from yesterday were run on a gel for evaluation and the result was that we had inserts of the correct length for ePRPRAT and ePRPRYT but not for ePRPRAT. | ||
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Digestion of PRKRYT 2 on AC (move to carrier plasmid), PRPRAT on AC (move to carrier plasmid) and PRPRYT AK (move to AC) | Digestion of PRKRYT 2 on AC (move to carrier plasmid), PRPRAT on AC (move to carrier plasmid) and PRPRYT AK (move to AC) | ||
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Latest revision as of 16:15, 21 October 2009
Evaluating in e.coli
The colony PCR from yesterday were run on a gel for evaluation and the result was that we had inserts of the correct length for ePRPRAT and ePRPRYT but not for ePRPRAT.
Various
BG-11 plates with kanamycin and without CuSO4 were also made for triparental mating.
--Anders 17:09, 11 October 2009 (UTC)
Transformation
Digestion of PRKRYT 2 on AC (move to carrier plasmid), PRPRAT on AC (move to carrier plasmid) and PRPRYT AK (move to AC)
