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Team:BIOTEC Dresden/Notebook1-1
From 2009.igem.org
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'''5th August''' | '''5th August''' | ||
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1. Electroporation of GB2005 cells have been done with DNA. | 1. Electroporation of GB2005 cells have been done with DNA. | ||
'''6th August''' | '''6th August''' | ||
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1. The plasmids containing RFP, GFP are visible under fluorescent microscope but the YFP and CFP constructs are not. So we continue with the plasmids pTetGFP, pTetmRFP, prom T7GFP, prpm T7Luc, pRedFlp(Rha Red-αβγ-tetFlp), pSC101-RhaFlp-Tet-Red-αβγ. | 1. The plasmids containing RFP, GFP are visible under fluorescent microscope but the YFP and CFP constructs are not. So we continue with the plasmids pTetGFP, pTetmRFP, prom T7GFP, prpm T7Luc, pRedFlp(Rha Red-αβγ-tetFlp), pSC101-RhaFlp-Tet-Red-αβγ. | ||
Latest revision as of 17:39, 21 October 2009
5th August
1. Electroporation of GB2005 cells have been done with DNA.
6th August
1. The plasmids containing RFP, GFP are visible under fluorescent microscope but the YFP and CFP constructs are not. So we continue with the plasmids pTetGFP, pTetmRFP, prom T7GFP, prpm T7Luc, pRedFlp(Rha Red-αβγ-tetFlp), pSC101-RhaFlp-Tet-Red-αβγ.
2. pTemRFP, promoter is visible after overnight incubation and also pTetGFP.
