Team:EPF-Lausanne

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{{EPF-Lausanne09}}
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<br><br>
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==Concept==
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[[Image:LovTAP_dimer.png|right|300px|thumb|LovTAP dimer bound to DNA]]
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Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.
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For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.
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[[Image:Mainpage.jpg|700 px|center]]
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The results clearly show that this light-induced gene switch tool works ''in vivo'', demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.
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[http://www.ubs.ch Main sponsor:]
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[[Image:Logo_UBS.jpg|link "www.ubs.com"|200 px|center]]
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==Last News==
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! scope=col | [[Image:Logo_Syngenta.png|80 px]]
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! scope=col | [[Image:Logo_UBS.jpg|200 px]]
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! scope=col | [[Image:Logo_ciba.jpg|80 px]]
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! scope=col | [[Image:Logo_nikon.jpg|60 px]]
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! scope=col | [[Image:Logo_tecan.gif|80 px]]
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|}
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'''Keep track with what we did so far'''
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''(12.07.09)''
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:This first week of wetlab we have done the following things
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*Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
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*Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
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*Orderd and received the primers needed for the PCR of LovTAP
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*Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
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*Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about this parts) into competent E.Coli
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*Fused the two BioBricks "LacI" and "term"
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*Digested the LovTAP PCR products and RBS part
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==Project Abstract==
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Recent discoveries of photoreceptors in many organisms gave us insights about a possible interest to use light responsive genetic tools in synthetic biology.
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More precisely, the final goal of our project is to induce a gene expression change, more specifically to turn on/off a gene, in a living organism in response to a light stimulus.
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We will use light sensitive DNA binding proteins, or light sensitive proteins that activate DNA binding proteins to transduce light input in a chosen output, for example reporter gene like GFP, RFP.
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The genetic circuits allowing us to measure the activity and the responsiveness of the light sensitive proteins are already designed, whereas parts and biobricks required to engineer these circuits are still in formation.
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If we demonstrate that this kind of light induced gene switch tool works in vivo, it would show that easier and faster tools could be used in several field of biology. It would induce more localized, more precise (time resolution) and drastically faster genetic changes than the current used tools, which will then allow research to evolve even better.
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</div><div CLASS="epfl09bouchon"></div>
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Latest revision as of 22:45, 21 October 2009



Mainpage.jpg



Concept


LovTAP dimer bound to DNA

Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.

For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.

The results clearly show that this light-induced gene switch tool works in vivo, demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.





Logo MerckSerono.png Logo Novartis.png Logo Syngenta.png Logo UBS.jpg Logo ciba.jpg Logo nikon.jpg Logo tecan.gif

Locations of visitors to this page

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