PCR inv

Kama

Tasks:

• Amplification of inv

Methods:

• PCR mixture's composition:

  2,5ul pfu buffer (Fermentas), 2,5ul MgSO4 (Fermentas), 1,5ul primers, 1ul dNTPs (10 mM)(new), 1ul template (Yersinia), 0,5ul pfu turbo polymerase (KNGiE), 1ul DMSO solution was topped up with H2O to 25ul.

• PCR programs:

inv

 4min 95°C 
 (30s 95°C, 1min 54°C , 4min15s 72°C)x31 
 10min 72°C 
 ~ 7°C

• Electrophoretic separation on 1% agarose gel



Results:


• Gel (from left)
1. GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
2. inv
3. inv control -



Notes:

• No product






Seba




Tasks:

• Transformation of E. coli DH5alfa



Methods:

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