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Team:Calgary/Modelling/Results
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The following is the graph produced by our differential equation based model under five different levels of LuxPQ (1, 10, 100, 1000, 10000): | The following is the graph produced by our differential equation based model under five different levels of LuxPQ (1, 10, 100, 1000, 10000): | ||
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| - | <center ><a href ="https://static.igem.org/mediawiki/2009/e/ec/IGEM_Calgary_Modelling_ChangesPQ_Oct20.jpg"> <img src = "https://static.igem.org/mediawiki/2009/e/ec/IGEM_Calgary_Modelling_ChangesPQ_Oct20.jpg" height="400px" width = " | + | <center ><a href ="https://static.igem.org/mediawiki/2009/e/ec/IGEM_Calgary_Modelling_ChangesPQ_Oct20.jpg"> <img src = "https://static.igem.org/mediawiki/2009/e/ec/IGEM_Calgary_Modelling_ChangesPQ_Oct20.jpg" height="400px" width = " 650px" alt =" click to view full size "> </a> </center> <br> |
<center> Figure 1: The rate of GFP degradation for different <i>LuxPQ</i> levels with respect to time.</center> | <center> Figure 1: The rate of GFP degradation for different <i>LuxPQ</i> levels with respect to time.</center> | ||
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Revision as of 02:24, 22 October 2009
UNIVERSITY OF CALGARY
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MODELLING INDEX
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DIFFERENTIAL EQUATIONS MODELLING RESULTS
The Effect of Variation of LuxPQ on the Production of GFP
The following is the graph produced by our differential equation based model under five different levels of LuxPQ (1, 10, 100, 1000, 10000):
 Green fluorescent protein (GFP) degradation rate remain relatively constant from one LuxPQ to 100 LuxPQ. This may be due to the amplifying effect of LuxPQ phosphatase activity. Since one LuxPQ have the potential to de-phosphorylate large amount of LuxU, having more LuxPQ around does not necessarily translate into faster de-phosphorylation of LuxU. Beyond 1,000 LuxPQ, however, the GFP degradation rate starts to fall. The reason behind this phenomenon could be that because the binding of AI-2 to LuxPQ is not 100%, not all of 1,000 LuxPQ are bound to 1,000 AI-2 molecules, leading to slower de-phosphorylation of LuxU. Having a slower de-phosphorylation rate of LuxU means that there are more than enough Luxu:pi and LuxO:pi to initiate the production of GFP, and therefore the degradation rate of GFP is slow or remain constant. |
