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Team:Washington/Notebook/Microscope
From 2009.igem.org
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==== Microscope ==== | ==== Microscope ==== | ||
| + | For cells: | ||
# Set up overnights of parts 48-51. Let grow overnight. | # Set up overnights of parts 48-51. Let grow overnight. | ||
# Dilute 1 ul overnight into 1ml | # Dilute 1 ul overnight into 1ml | ||
| - | # Add 1 mm IPTG and let grow for four hours | + | # Add 1 mm IPTG and let grow for four hours at 37 C. |
| - | # After cells have grown up, | + | # After cells have grown up, spin the cells down. |
| - | # | + | # Take cell pellet, and then dilute in 1ml molecular grade water. |
| + | # Add in flourophore (at a 1 uM concentration) and allow time to bind (1 hour). | ||
| + | # Look at cells under the microscope. | ||
| + | |||
| + | For beeds: | ||
| + | # Place 1 ul beads in 1 ml along with 1 ul flourophore. | ||
# Allow beads to bind to flourophore, then spin beads down, remove supernatent and replace with 1 ml water. | # Allow beads to bind to flourophore, then spin beads down, remove supernatent and replace with 1 ml water. | ||
| - | # | + | # Look at the beads under the microscope. |
{{Template:Team:Washington/Templates/Footer}} | {{Template:Team:Washington/Templates/Footer}} | ||
Revision as of 02:50, 22 October 2009
Microscope
For cells:
- Set up overnights of parts 48-51. Let grow overnight.
- Dilute 1 ul overnight into 1ml
- Add 1 mm IPTG and let grow for four hours at 37 C.
- After cells have grown up, spin the cells down.
- Take cell pellet, and then dilute in 1ml molecular grade water.
- Add in flourophore (at a 1 uM concentration) and allow time to bind (1 hour).
- Look at cells under the microscope.
For beeds:
- Place 1 ul beads in 1 ml along with 1 ul flourophore.
- Allow beads to bind to flourophore, then spin beads down, remove supernatent and replace with 1 ml water.
- Look at the beads under the microscope.
