Team:BIOTEC Dresden/Notebook Vesicles

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=== Gene Expression in vesicles ===
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=== Vesicle formation in a microfluidic chamber ===
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<br>
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The first step was to create a microfluidic chamber that creates vesicles of homogenous size. In the second step, a commercial gene expression kit was introduced into the vesicles and tested using a GPF plasmid.
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'''Strategy'''
'''Strategy'''
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Vesicles are formed using a microfluidic system, allowing for small reaction volumes and a controllable environment. The system is used to create emulsions of material in aqueous phase in a lipid phase. Proteins and various buffers are eventually added to the aqueous phase to carry out transcription and translation of the protein of interest in the vesicles, in this case, FLP recombinase. The production of FLP can be checked by tagging the final protein with a fluorescent protein like GFP, which can be then viewed using fluorescent microscopy.
Vesicles are formed using a microfluidic system, allowing for small reaction volumes and a controllable environment. The system is used to create emulsions of material in aqueous phase in a lipid phase. Proteins and various buffers are eventually added to the aqueous phase to carry out transcription and translation of the protein of interest in the vesicles, in this case, FLP recombinase. The production of FLP can be checked by tagging the final protein with a fluorescent protein like GFP, which can be then viewed using fluorescent microscopy.
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'''[[Team:BIOTEC_Dresden/Methods_Vesicles|Methods]]'''
'''[[Team:BIOTEC_Dresden/Methods_Vesicles|Methods]]'''
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The protocols used to create the microfluidic chamber and express genes in it are given here.
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This is how we created vesicles in a microfluidic chamber.
'''[[Team:BIOTEC_Dresden/Results_Vesicles|Results]]'''
'''[[Team:BIOTEC_Dresden/Results_Vesicles|Results]]'''
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Following that protocol, chambers were produced and gene expression was attempted. Here are the results obtained during that process.
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Here are the results for the vesicle formation in a microfluidic chamber.
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Latest revision as of 03:41, 22 October 2009

Vesicle formation in a microfluidic chamber


Strategy

Vesicles are formed using a microfluidic system, allowing for small reaction volumes and a controllable environment. The system is used to create emulsions of material in aqueous phase in a lipid phase. Proteins and various buffers are eventually added to the aqueous phase to carry out transcription and translation of the protein of interest in the vesicles, in this case, FLP recombinase. The production of FLP can be checked by tagging the final protein with a fluorescent protein like GFP, which can be then viewed using fluorescent microscopy.


Methods

This is how we created vesicles in a microfluidic chamber.


Results

Here are the results for the vesicle formation in a microfluidic chamber.

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