Team:PKU Beijing

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{{PKU_Beijing/Sidebar}}
{{PKU_Beijing/Sidebar}}
{{PKU_Beijing/Header2}}
{{PKU_Beijing/Header2}}
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==='''Project Abstract'''===
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==='''Conditioned Reflex Mimicking in ''E.coli'''''===
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We are engineering our E. coli cells to process the correlation information of two enviornmental signal, similar to the process of conditioning in higher orgamisms. We have constructed and tested a series of AND gates which can sense the two signals: the conditioned and unconditioned stimuli. With the presence of both signals, the AND gate outputs a repressor protein and then changes the state of the bistable switch, which acts as a memory module. In this way, our E. coli cells can convert the information about the concurrence of the two signals into its memory. After the memory module is switched and given the "conditioned stimulus", the E. coli cells will pass the information to the reporter module and thus exhibit the "conditioned response."
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We are engineering our E.coli cells to process the correlation information of two environmental signal, similar to the process of classical conditioning in higher organisms. In our circuits we use a bistable switch as the memory module to represent the memory state; we also constructed a series of AND gates which can sense conditioned and unconditioned signals, and output a repressor protein to change the memory state of the bistable switch. In this way, our E.coli cells can convert the information on the concurrence of two signals to its memory. After the memory module is switched, given the conditioned stimulus, E.coli will activate its reporter module and thus exhibit the "conditioned response".
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[[Image:PKU_Logic_Circuit.png|600px|center]]
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For more details go to the [[Team:PKU_Beijing/Project|Project]] page.
 
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==='''Peking University'''===
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[[Team:PKU_Beijing/Project|Start your journey into the fancy world of Conditioned Reflex Mimicking in E.coli]]
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Peking University is a comprehensive and national key university. The campus, known as "Yan Yuan"(the garden of Yan), is situated at Haidian District in the western suburb of Beijing, with a total area of 2,743,532 square metres (or 274 hectares). It stands near to the Yuanmingyuan Garden and the Summer Palace.
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Peking University is proud of its outstanding faculty, including 53 members of the Chinese Academy of Sciences (CAS), 7 members of the Chinese Academy of Engineering (CAE), and 14 members of the Third World Academy of Sciences (TWAS).
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==='''Road Map'''===
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Following is the road map of our project. We still need a second stage assembly in order to finish our project.
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You can click on the pictures to see the '''results''' of each module.So here it is.
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{{PKU_Beijing/RoadMap}}
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The university has effectively combined research on important scientific subjects with the training of personnel with a high level of specialized knowledge and professional skill as demanded by the country's socialist modernization. It strives not only for improvements in teaching and research work, but also for the promotion of interaction and mutual promotion among various disciplines.
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==='''Recommondations For Our Site'''===
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*'''Human Practice'''<BR>
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You would really want to read our report from a survey we carried out on DIYbio.
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Many similar surveys focus on what people think about, and what people would '''possibly''' do if faced with such problems. Our survey shows what people '''really''' do to DIYbio. It directly reflects the reality of the present situation that DIYbio is in.<br>
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[[Team:PKU_Beijing/Human/Survey|'''[Learn More]''']].
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Thus Peking University has become a center for teaching and research and a university of a new type, embracing diverse branches of learning such as basic and applied sciences, social sciences and the humanities, and sciences of medicine, management, and education. Its aim is to rank among the world's best universities in the future.
 
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==='''Acknowledgement'''===
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*'''Improvement on AND Gate'''<BR>
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The PKU iGEM2009 Team strongly acknowledges following institutes/companies
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Our original AND Gate is from Voigt Lab. We make it into standard parts and use a combination of promoter, which leads to several AND Gates that has different properties. The most wonderful thing in the summer holiday that we achieved a greatly improved AND Gate.<br>
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<html>
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[[Team:PKU_Beijing/Parts_Characterization/BBa_K228258|'''[Learn More]''']].
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<a href="http://www.bio.pku.edu.cn/syzx/zhongxin/zhongxin.htm"><img src=https://static.igem.org/mediawiki/2008/2/25/PKU_Teaching_Center_Experimental_Biology.png width="500" height="85"></a><p>
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<a href="http://ctb.pku.edu.cn/main/cn/index.htm"><img src=https://static.igem.org/mediawiki/2009/6/6e/PKU_CTB.gif width="500" height="61"></a><p>
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</html>
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We also want to thank the following Professors of the Life Science School, for supporting us with equipments and reagents.
 
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*'''Assembly'''<BR>
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<html>
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The most difficult work in the construction of a multi-Module system is assembly. After we have accomplished all the individual modules from Jul to Sept, we made a breakthrough during the National Day (we did experiments during the holiday). That is the first stage assembly is accomplished.<br>
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<A href="http://www.bio.pku.edu.cn/faculty/chenjg/">Prof. Jianguo Chen</A>
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[[Team:PKU_Beijing/Project/Assemble|'''[Learn More]''']].
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<A href="http://www.bio.pku.edu.cn/faculty/liud/">Prof. Dong Liu</A>
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<A href="http://www.bio.pku.edu.cn/faculty/wangsq/">Prof. Shiqiang Wang</A>
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<A href="http://www.bio.pku.edu.cn/faculty/wangyp/">Prof. Yiping Wang</A>
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<A href="http://www.bio.pku.edu.cn/faculty/yulc/">Prof. Longchuan Yu</A>
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<A href="http://www.bio.pku.edu.cn/faculty/zhangy/">Prof. Yan Zhang</A>
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</html>
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{{PKU_Beijing/Foot}}
{{PKU_Beijing/Foot}}
__NOTOC__
__NOTOC__

Latest revision as of 12:13, 22 May 2010

News

  • We will bring our seal to jamboree, also Chinese knots as souvenir.
PKU Seal.png
  • Our T-shirt has come out:
  • First Stage Assembly accomplished, moving on!
  • Assembly between the first two Modules has been recently accomplished by Shuke Wu! More detailed work is being carried on by him!
  • Our new site is up! Feel free to send any comment to c.z.tian AT gmail.com

Countdown




Visitors

Locations of visitors to this page

 

Conditioned Reflex Mimicking in E.coli

We are engineering our E.coli cells to process the correlation information of two environmental signal, similar to the process of classical conditioning in higher organisms. In our circuits we use a bistable switch as the memory module to represent the memory state; we also constructed a series of AND gates which can sense conditioned and unconditioned signals, and output a repressor protein to change the memory state of the bistable switch. In this way, our E.coli cells can convert the information on the concurrence of two signals to its memory. After the memory module is switched, given the conditioned stimulus, E.coli will activate its reporter module and thus exhibit the "conditioned response".

PKU Logic Circuit.png


Start your journey into the fancy world of Conditioned Reflex Mimicking in E.coli

Road Map

Following is the road map of our project. We still need a second stage assembly in order to finish our project. You can click on the pictures to see the results of each module.So here it is.

Recommondations For Our Site

  • Human Practice

You would really want to read our report from a survey we carried out on DIYbio. Many similar surveys focus on what people think about, and what people would possibly do if faced with such problems. Our survey shows what people really do to DIYbio. It directly reflects the reality of the present situation that DIYbio is in.
[Learn More].


  • Improvement on AND Gate

Our original AND Gate is from Voigt Lab. We make it into standard parts and use a combination of promoter, which leads to several AND Gates that has different properties. The most wonderful thing in the summer holiday that we achieved a greatly improved AND Gate.
[Learn More].


  • Assembly

The most difficult work in the construction of a multi-Module system is assembly. After we have accomplished all the individual modules from Jul to Sept, we made a breakthrough during the National Day (we did experiments during the holiday). That is the first stage assembly is accomplished.
[Learn More].




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