Virginia Commonwealth/13 July 2009
From 2009.igem.org
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* Perform Electrophoresis test on Digested parts J23106 and J06702 | * Perform Electrophoresis test on Digested parts J23106 and J06702 | ||
* Ligate parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone) | * Ligate parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone) | ||
- | * Transform ligated DNA into e. coli | + | * Transform ligated DNA into e. coli NEB 10 beta |
[[User:Trentay|Trentay]] 18:34, 13 July 2009 (UTC) | [[User:Trentay|Trentay]] 18:34, 13 July 2009 (UTC) | ||
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''Maria and Afton'' | ''Maria and Afton'' | ||
* Gel Electrophoresis | * Gel Electrophoresis | ||
- | * | + | * Digestion of J23100 |
- | [[User:Trentay|Trentay]] | + | * Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone) |
+ | * Plated: | ||
+ | ** (+) Control: NEB 10 beta on LB | ||
+ | ** (+) Control: NEB 10 beta on LB shocked | ||
+ | ** (-) Control: NEB 10 beta on Cm | ||
+ | ** NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5) | ||
+ | [[User:Trentay|Trentay]] 20:26, 13 July 2009 (UTC) |
Revision as of 20:26, 13 July 2009
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Contents |
Monday 13 July 2009
Results
Maria and Afton
- Digestion was successful on parts J23106 and J06702
Trentay 18:30, 13 July 2009 (UTC)
Tasks
Maria and Afton
- Make daily planner for experimentation
- Perform Electrophoresis test on Digested parts J23106 and J06702
- Ligate parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
- Transform ligated DNA into e. coli NEB 10 beta
Trentay 18:34, 13 July 2009 (UTC)
Kevin and Adam
- Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock.
- 2
Wetlab
Maria and Afton
- Gel Electrophoresis
- Digestion of J23100
- Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
- Plated:
- (+) Control: NEB 10 beta on LB
- (+) Control: NEB 10 beta on LB shocked
- (-) Control: NEB 10 beta on Cm
- NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5)
Trentay 20:26, 13 July 2009 (UTC)