13th July

Weenink

prepared 10 agar+amp plates

Also did the first transformations to see how it goes:

BBa_K145280 (GFP-LVA + TetR generators)
BBa_K145279 (GFP + TetR generators)

4 vials of DH5alpha comptetent cells were used (2 for each biobrick)

after addition of 1 µl rehydrated biobrick DNA, the tubes were incubated for approx 15 (±5) min. on ice. Then one tube of each transformation was heatshocked for 1 min at 42 degrees.

All four transformations were plated out and put at 37 degrees (at 19:00)
BBa_K145280 no heatshock approx 80 microliter on agar+amp plate
BBa_K145279 no heatshock 50 microliter on agar+amp plate
BBa_K145280 heatshocked 50 microliter on agar+amp plate
BBa_K145279 heatshocked 50 microliter on agar+amp plate

Modelling

Worked on the delay device modelling (established the ODE's for a negative feedforward system using the Bologna 2008 project)

Searched for some articles regarding conjugation modelling, and found two:

• [http://www3.interscience.wiley.com/journal/118837812/abstract?CRETRY=1&SRETRY=0 A model for bacterial conjugal gene transfer on solid surfaces] and [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2K-4PT7X7G-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=fcacb99317376ced3df42e7e8178fc78 Rule-based modelling of conjugative plasmid transfer and incompatibility ]