Team:EPF-Lausanne/Team
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== '''What we want to do''' == | == '''What we want to do''' == | ||
- | Light-sensitive proteins can easily be found in nature. | + | Light-sensitive proteins can easily be found in nature, but they have never been cloned into other cells. |
In this project, our aim is to design a fusion/hybrid protein that would allow genetic regulation through light control. | In this project, our aim is to design a fusion/hybrid protein that would allow genetic regulation through light control. | ||
- | Therefore we are working on cloning strategies that would allow us to fuse natural "wild" light-sensitive domains with regulatory proteins. The idea is to allow transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain. This transmitted conformational change would then result in an increase or decrease of the regulatory domain's affinity for the DNA promoter site. | + | Therefore we are working on cloning strategies that would allow us to fuse natural "wild" light-sensitive domains with regulatory proteins. The idea is to allow transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain. This transmitted conformational change would then result in an increase or decrease of the regulatory domain's affinity for the DNA promoter site. |
The overal effect would thus be a genetic expression controlled by light! | The overal effect would thus be a genetic expression controlled by light! | ||
- | + | To improve the change induced by light (that is generally very unstable), we also plan a modeling part where the aim is to find which residue we would have to mutate in order to have a stable protein after the switch. | |
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== '''Where we are from''' == | == '''Where we are from''' == |
Revision as of 12:20, 21 July 2009
Who we are
Advisors:
|
What we want to do
Light-sensitive proteins can easily be found in nature, but they have never been cloned into other cells.
In this project, our aim is to design a fusion/hybrid protein that would allow genetic regulation through light control.
Therefore we are working on cloning strategies that would allow us to fuse natural "wild" light-sensitive domains with regulatory proteins. The idea is to allow transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain. This transmitted conformational change would then result in an increase or decrease of the regulatory domain's affinity for the DNA promoter site.
The overal effect would thus be a genetic expression controlled by light!
To improve the change induced by light (that is generally very unstable), we also plan a modeling part where the aim is to find which residue we would have to mutate in order to have a stable protein after the switch.
Where we are from
We are all studying at EPFL aka the Swiss Institute of Technology of Lausanne. The campus is located near the shore of lake Geneva in the surroundings of the city of Lausanne, 50 km away from Geneva.