EPF-Lausanne/7 July 2009

From 2009.igem.org

(Difference between revisions)
(→People in the lab)
(→People in the lab)
Line 73: Line 73:
</body>
</body>
</html>
</html>
 +
 +
<br>
 +
 +
<html>
 +
<head>
 +
<title>Texte du titre</title>
 +
</head>
 +
<body>
 +
<h1>Liens sous une autre forme</h1>
 +
<form action="bouton.htm">
 +
<div>
 +
<button name="cliquemoi" type="button"
 +
  value="SELFHTML" onClick="self.location.href='http://actuel.fr.selfhtml.org/'">
 +
<p><img src="selfhtml.gif" width="106" height="109" border="0" alt="SELFHTML"><br>
 +
<b>page d'accueil de SELFHTML</b></p>
 +
</button>
 +
</div>
 +
</form>
 +
</body>
 +
</html>
 +

Revision as of 07:58, 28 July 2009



Contents

7 July 2009



Wet Lab

We have to grow the 3 strains generously sent by Tom Beatty

The three strains are :

  • R.Palustris CEA001 (wild type) ; should be grown on LB medium only
  • R.Palustris BPHP1+ ; should be grown on LB with gentamycin (100 micrograms/ml)
  • E.Coli DH10B (pBPH/hmu0) ; should be grown on LB with gentamycin (20 micorgrams/ml)


The transformed LOVTAP and TrpR worked well (N.B. the plasmid of TrpR is pUC19 so the antibiotic resistance is Amp -> see below)


pUC19 plasmid


We did the glycerol stock, located in the -80 fridge, first floor of the iGEM compartement.

Then, a miniprep was done with both cultures. A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.


Cloning Strategy

To design plasmids : software Vector NTI


People in the lab

Tu, Heidi, Rafael, Basile, Nath





Texte du titre

Liens sous une autre forme





Retrieved from "http://2009.igem.org/EPF-Lausanne/7_July_2009"