Team:Cambridge/Project/Violacein
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+ | = Violacein Pigment = | ||
+ | |||
+ | |||
+ | == Introduction == | ||
+ | |||
+ | The Violaceein pigment is produced from L-tryptophan via an pathway involving four enzymes, vioA-D. This scheme is shown below: | ||
+ | |||
+ | [[Image:Violacein pigment production.jpg]] | ||
+ | |||
+ | As module 5 is Aqua, this gives us two different colour controlled by the four genes allowing the construction of logic gates. | ||
+ | |||
+ | == Research by previous iGem teams == | ||
+ | Previous biobricks: | ||
+ | |||
+ | {| style="color:#CCC; background-color:#3D5089;" cellpadding="6" cellspacing="0" border="1" | ||
+ | ! Registry Code | ||
+ | ! Team | ||
+ | ! Sequence Description | ||
+ | ! Notes | ||
+ | |- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1" | ||
+ | | | ||
+ | | | ||
+ | | | ||
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+ | |- style="color:#333; background-color:#A3C3FF;" cellpadding="6" cellspacing="0" border="1" | ||
+ | | | ||
+ | | | ||
+ | | | ||
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+ | |} | ||
+ | |||
+ | == Action plan of our team == | ||
+ | |||
+ | Our action plan is as follows: | ||
+ | |||
+ | :1. | ||
+ | |||
+ | == Test activity of X == | ||
+ | |||
+ | '''Mapping of Biobrick X''': | ||
+ | (image) | ||
+ | |||
+ | == Construct composite Biobrick X == | ||
+ | |||
+ | '''Plan mapping of X''': | ||
+ | |||
+ | (image) | ||
+ | |||
+ | == Test colour changes by X == | ||
+ | |||
+ | == Characterisation of Biobricks and colour output == | ||
+ | |||
+ | == Test compatibility with other biobricks == | ||
+ | |||
+ | |||
+ | <!--Do not remove the first and last lines in this page!--><div id="contentbox_bottom"></div></div> |
Revision as of 11:13, 29 July 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Violacein Pigment
Introduction
The Violaceein pigment is produced from L-tryptophan via an pathway involving four enzymes, vioA-D. This scheme is shown below:
As module 5 is Aqua, this gives us two different colour controlled by the four genes allowing the construction of logic gates.
Research by previous iGem teams
Previous biobricks:
Registry Code | Team | Sequence Description | Notes |
---|---|---|---|
Action plan of our team
Our action plan is as follows:
- 1.
Test activity of X
Mapping of Biobrick X: (image)
Construct composite Biobrick X
Plan mapping of X:
(image)