Virginia Commonwealth/21 May 2009
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==Thursday 21 May 2009== | ==Thursday 21 May 2009== | ||
===Tasks=== | ===Tasks=== | ||
- | + | #Make stock of electrocompetent ''E. coli'' K-12 | |
- | + | #Transform ''E. coli'' with the following: | |
+ | ##BBa_I13521 (RPF PRET, AmpR) - located in plate 2, well 6O | ||
+ | ##BBa_I13522 (GRP PRET, AmpR) - located in plate 2, well 8A | ||
+ | ##BBa_E0840 (GFP RET, AmpR) - located in plate 1, well 12O | ||
+ | ##BBa_R0040 (Promoter) - located in plate 1, well 6I | ||
+ | ##pSB1AK3-J04450 (KanR plasmid w/ RPF insert) - located in palte 1, well 13A | ||
+ | ##BBa_E0240 (GFP RET, AmpR) - located in plate 1, well 12M) | ||
===Results=== | ===Results=== |
Revision as of 15:04, 21 May 2009
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Thursday 21 May 2009
Tasks
- Make stock of electrocompetent E. coli K-12
- Transform E. coli with the following:
- BBa_I13521 (RPF PRET, AmpR) - located in plate 2, well 6O
- BBa_I13522 (GRP PRET, AmpR) - located in plate 2, well 8A
- BBa_E0840 (GFP RET, AmpR) - located in plate 1, well 12O
- BBa_R0040 (Promoter) - located in plate 1, well 6I
- pSB1AK3-J04450 (KanR plasmid w/ RPF insert) - located in palte 1, well 13A
- BBa_E0240 (GFP RET, AmpR) - located in plate 1, well 12M)