Revision as of 07:42, 31 July 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Individual parts

Short description	Resistance	Status	Location	Additional notes
Ribokey	A/A	ordered at Geneart
antikey	A/A	ordered at geneart
lock	Ap	ordered at geneart
Terminator	Ak	Available here	-80
RBS	Ap	Available here	-80
promotor	Ap	Available here	-80
Sam8		ordered		not in distribution, only in Edinburgh 2007 submissions
Sam5		ordered		not in distribution, only in Edinburgh 2007 submissions
comt	A/A	in registry	in registry
fcs		mail sent		gene from http://www.ncbi.nlm.nih.gov/pubmed/14602615 1, http://www.springerlink.com/content/bj47xnqn5kr65wvp/fulltext.pdf 2 and http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17437627#id538997 3
ech		mail sent		gene from http://www.ncbi.nlm.nih.gov/pubmed/14602615 1, http://www.springerlink.com/content/bj47xnqn5kr65wvp/fulltext.pdf 2 and http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17437627#id538997 3
Vanillin Binding protein	AT	mailed Valencia Univ.		sequence inconsistent on partsregistry
Trg envZ fusion protein	K	mailed Valencia Univ.		sequence inconsistent on partsregistry
ompF promotor	A
YcgF/E promotor	Primers chosen			blue light promotor from http://www.ncbi.nlm.nih.gov/pubmed/19240136 1

Short description	Status	Additional notes
OmpF promotor + key	Biobrick made
Blr promotor + key	Biobrick made
Vanillin receptor	Biobrick made
Vanillin synthesis	Biobrick made	5 paths in pathway never tested together in E. Coli
Short Vanillin synthesis	Biobrick made	Short version containing only the last 2 parts of the pathway, these have been tested in E. Coli

@@ Line 7: / Line 7: @@
 !| Partnumber||Short description|| Resistance || Status||Location||Additional notes
 |- align="center"
-| {{kulpart|BBa_J23066|icon}} ||Ribokey|| A/A ||ordered at Geneart|| -80, F1-72 ||
+| {{kulpart|BBa_J23066|icon}} ||Ribokey|| A/A ||ordered at Geneart||  ||
 |- align="center"
-| {{kulpart|BBa_K238005|icon}}|| antikey || ||  Biobrick made  || ||
+| {{kulpart|BBa_K238005|icon}}|| antikey || A/A ||  ordered at geneart  || ||
 |-align="center"
-| {{kulpart|BBa_J23078|icon}}|| lock || Ap || Available here || -80, F1-41 ||
+| {{kulpart|BBa_J23078|icon}}|| lock || Ap || ordered at geneart ||  ||
 |-align="center"
 | {{kulpart|BBa_B0015|icon}}  ||Terminator|| Ak || Available here|| -80  ||
 |-align="center"
-| {{kulpart|BBa_B0032|icon}}|| RBS || Ap || Available here || ||
+| {{kulpart|BBa_B0032|icon}}|| RBS || Ap || Available here || -80 ||
 |-align="center"
-| {{kulpart|BBa_J23110|icon}}|| promotor || Ap || Available here || ||
+| {{kulpart|BBa_J23110|icon}}|| promotor || Ap || Available here || -80 ||
 |-align="center"
 | {{kulpart|BBa_I742142|icon}}|| Sam8 || || ordered || || not in distribution, only in Edinburgh 2007 submissions