Team:KULeuven/Used Parts

From 2009.igem.org

(Difference between revisions)
(→Individual parts)
(→Individual parts)
Line 21: Line 21:
| {{kulpart|BBa_I742142|icon}}|| Sam8 || || received from Edinburgh || ||   
| {{kulpart|BBa_I742142|icon}}|| Sam8 || || received from Edinburgh || ||   
|-align="center"
|-align="center"
-
| {{kulpart|BBa_I742141|icon}}|| Sam5 || || received from Edinburgh || ||  
+
| {{kulpart|BBa_K238007|icon}}|| Sam5 || || received from Edinburgh || ||  
|-align="center"
|-align="center"
| {{kulpart|BBa_I742107|icon}}|| comt || A/A || received from Edinburgh || ||  
| {{kulpart|BBa_I742107|icon}}|| comt || A/A || received from Edinburgh || ||  
|-align="center"
|-align="center"
-
| {{kulpart|BBa_I742115|icon}}|| fcs || || mail sent || || gene from
+
| {{kulpart|BBa_I742115|icon}}|| fcs || || received from Edinburgh || ||
|-align="center"
|-align="center"
-
| {{kulpart|BBa_I742113|icon}}|| ech || || mail sent || || gene from
+
| {{kulpart|BBa_I742113|icon}}|| ech || || received from Edinburgh || ||  
|-align="center"
|-align="center"
-
| {{kulpart|BBa_J58105|icon}}|| Vanillin Binding protein || AT || mailed Valencia Univ. || || sequence inconsistent on partsregistry
+
| {{kulpart|BBa_K238008|icon}}|| VirA receptor || || primers ordered || || This is purified from Agrobacterium temefaciens
|-align="center"
|-align="center"
-
| {{kulpart|BBa_J58104|icon}}|| Trg envZ fusion protein || K || mailed Valencia Univ. || || sequence inconsistent on partsregistry
+
| {{kulpart|BBa_K238009|icon}}|| VirG activator || || primers ordered || || This is purified from Agrobacterium tumefaciens
|-align="center"
|-align="center"
-
| {{kulpart|BBa_R0084|icon}}|| ompF promotor || A || || ||
+
| {{kulpart|BBa_K23010|icon}}|| RpoA  || || primers ordered || || This is purified from Agrobacterium tumefaciens and is necessary for VirG to work in E.coli
|-align="center"
|-align="center"
-
| {{kulpart|BBa_K238000|icon}}|| YcgF/E promotor || Primers chosen || || || blue light promotor from [[http://www.ncbi.nlm.nih.gov/pubmed/19240136 1]]
+
| {{kulpart|BBa_K238011|icon}}|| VirB promoter region  || || primers ordered || || This is purified from Agrobacterium tumefaciens and has virboxes that bind VirG
 +
|-align="center"
 +
| {{kulpart|BBa_K238000|icon}}|| YcgF/E promotor || region purified, actual promoter identified|| || || blue light promotor from [[http://www.ncbi.nlm.nih.gov/pubmed/19240136 1]]

Revision as of 08:23, 31 July 2009

Individual parts

PartnumberShort description Resistance StatusLocationAdditional notes
Ribokey A/A ordered at Geneart
antikey A/A ordered at geneart
lock Ap ordered at geneart
Terminator Ak Available here -80
RBS Ap Available here -80
promotor Ap Available here -80
Sam8 received from Edinburgh
Sam5 received from Edinburgh
comt A/A received from Edinburgh
fcs received from Edinburgh
ech received from Edinburgh
VirA receptor primers ordered This is purified from Agrobacterium temefaciens
VirG activator primers ordered This is purified from Agrobacterium tumefaciens
RpoA primers ordered This is purified from Agrobacterium tumefaciens and is necessary for VirG to work in E.coli
VirB promoter region primers ordered This is purified from Agrobacterium tumefaciens and has virboxes that bind VirG
YcgF/E promotor region purified, actual promoter identified blue light promotor from http://www.ncbi.nlm.nih.gov/pubmed/19240136 1


Composite parts

PartnumberShort description Resistance StatusLocationAdditional notes
OmpF promotor + key Biobrick made
Blr promotor + key Biobrick made
Vanillin receptor Biobrick made
Vanillin synthesis Biobrick made 5 paths in pathway never tested together in E. Coli
Short Vanillin synthesis Biobrick made Short version containing only the last 2 parts of the pathway, these have been tested in E. Coli
Retrieved from "http://2009.igem.org/Team:KULeuven/Used_Parts"