From 2009.igem.org
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| | ===Transporters=== | | ===Transporters=== |
| | + | GlpF |
| | + | PCR 2 was add on gel again as noted below at accumulation. |
| | + | |
| | + | PCR 1 was |
| | + | '''GlpF PCR1''' |
| | + | {| |
| | + | | |
| | + | <!--Tabel 1 hier--> |
| | + | |
| | + | {| border="1" |
| | + | |+ '''PCR1''' |
| | + | ! Component !! amount |
| | + | |- |
| | + | ! 2x Phusion MM |
| | + | | 12.5 uL |
| | + | |- |
| | + | ! MQ |
| | + | | 10.25 uL |
| | + | |- |
| | + | ! GlpF Fw |
| | + | | 1 uL |
| | + | |- |
| | + | ! GlpF MutRev |
| | + | | 1 uL |
| | + | |- |
| | + | ! DNA GlpF Full Lenght (extracted as noted below at accumulation)(not added to tube nr2) |
| | + | | 0.25 uL |
| | + | |} |
| | + | |
| | + | |width="10"| |
| | + | | |
| | + | <!--Tabel 2 hier--> |
| | + | {| |
| | + | ! GlpF PCR1 program |
| | + | ! Temperature |
| | + | ! Time |
| | + | |- |
| | + | |Denaturing |
| | + | |98° |
| | + | |2.00 min |
| | + | |- |
| | + | |Start Cycles 30X |
| | + | |- |
| | + | |Denaturing |
| | + | |98° |
| | + | |10 sec |
| | + | |- |
| | + | |Annealing |
| | + | |60° |
| | + | |10 sec |
| | + | |- |
| | + | |Elongation |
| | + | |72° |
| | + | |10 sec |
| | + | |- |
| | + | | |
| | + | |End cycles |
| | + | |- |
| | + | |Final elongation |
| | + | |72° |
| | + | |5 min |
| | + | |- |
| | + | |Hold |
| | + | |4° |
| | + | |Forever |
| | + | |} |
| | + | |width="10"| |
| | + | | |
| | + | |} |
| | + | |
| | + | A 2% agarose gel was used, a band of 105 bp was expected. |
| | + | |
| | + | [[Image:F102471_2009-07-27_16hr_21min_GlpFPCR1_noted.JPG]] |
| | + | |
| | + | The band in row 2 was cut and purified using the zymo research Zymoclean TM Gel DNA Recovery Kit. |
| | + | |
| | + | The purified PCR1 DNA and PCR2 DNA were used to run PCR3 |
| | + | |
| | + | |
| | + | '''GlpF PCR3''' |
| | + | {| |
| | + | | |
| | + | <!--Tabel 1 hier--> |
| | + | |
| | + | {| border="1" |
| | + | |+ '''PCR1.1''' |
| | + | ! Component !! amount |
| | + | |- |
| | + | ! 2x Phusion MM |
| | + | | 12.5 uL |
| | + | |- |
| | + | ! GlpF PCR1 |
| | + | | 6 uL |
| | + | |- |
| | + | ! GlpF PCR2 |
| | + | | 6 uL |
| | + | |} |
| | + | |
| | + | |width="10"| |
| | + | | |
| | + | <!--Tabel 2 hier--> |
| | + | {| |
| | + | ! GlpF PCR1 program |
| | + | ! Temperature |
| | + | ! Time |
| | + | |- |
| | + | |Denaturing |
| | + | |98° |
| | + | |2.00 min |
| | + | |- |
| | + | |Start Cycles 30X |
| | + | |- |
| | + | |Denaturing |
| | + | |98° |
| | + | |10 sec |
| | + | |- |
| | + | |Annealing |
| | + | |60° |
| | + | |10 sec |
| | + | |- |
| | + | |Elongation |
| | + | |72° |
| | + | |30 sec |
| | + | |- |
| | + | | |
| | + | |End cycles |
| | + | |- |
| | + | |Final elongation |
| | + | |72° |
| | + | |5 min |
| | + | |- |
| | + | |Hold |
| | + | |4° |
| | + | |Forever |
| | + | |} |
| | + | |width="10"| |
| | + | | |
| | + | |} |
| | + | The results of the 1% agarose gel can be found at 28/07/09 |
| | | | |
| | ===Metal Accumulation=== | | ===Metal Accumulation=== |
| | | | |
| - | ArsR PCR
| |
| | | | |
| - | Gel with ArsR PCR with Taq, Taq(no DNA), Phusion, - , Glpf PCR2, GlpF PCR1, Glpf full lenght
| |
| | | | |
| - | [[Image:270709_ArsR+GlpF.jpg]] | + | Gel with ArsR PCR((24/07/09) |
| | + | |
| | + | [[Image:270709_ArsR+GlpF.JPG]] |
| | + | |
| | + | ArsR/GlpF gel extraction |
| | + | |
| | + | :: - Used zymo research Zymoclean TM Gel DNA Recovery Kit to extract ArsR, GlpF PCR2 and GlpF FullLenght from gel (cut from gel as shown in figure above). |
| | + | |
| | + | '''GlpF PCR1''' |
| | + | |
| | + | {| |
| | + | PCR Mix |
| | + | |12,5 uL |
| | + | |Phusion |
| | + | |- |
| | + | |1 uL |
| | + | |GlpF FW primer |
| | + | |- |
| | + | |1 uL |
| | + | |GlpF RevMut primer |
| | + | |- |
| | + | |0,25 uL |
| | + | |GlpF Full Lenght DNA (extracted as noted above)(not in tube2) |
| | + | |- |
| | + | |10,25 uL |
| | + | |MQ |
| | + | |} |
| | | | |
| | ===Vectors=== | | ===Vectors=== |
| | + | |
| | + | *'''Restriction digest on pSB3K3-const. promoters and pSB1AC3-cons. promoters and pSB1A2-lac promoter''' |
| | + | **Plasmids isolated with sigma plasmid isolation kit from o/n culture 24-25 July |
| | + | **Restriction was done with 250-500ng plasmid |
| | + | |
| | + | {| |
| | + | | |
| | + | <!--Tabel 1 hier--> |
| | + | |width="10"| |
| | + | | |
| | + | |
| | + | {| border="1" |
| | + | '''Check pLac insert''' |
| | + | |
| | + | |10x Tango buffer |
| | + | |2 uL |
| | + | |- |
| | + | |pSB1A2-lac1&2 (19-22 ng/uL) |
| | + | |17 uL |
| | + | |- |
| | + | |EaeI (CfrI) |
| | + | |1 uL |
| | + | |- |
| | + | |Total |
| | + | |20 uL |
| | + | |- |
| | + | |} |
| | + | |
| | + | <!--Tabel 2 hier--> |
| | + | |width="10"| |
| | + | | |
| | + | |
| | + | {| border="1" |
| | + | '''Check const. promoter insert (without promoter single cut, with 2x''' |
| | + | |
| | + | |10x Orange buffer |
| | + | |2 uL |
| | + | |- |
| | + | |pSB3K3-HML or pSB1AC3-HML |
| | + | |7 or 17 uL (depending on conc.) |
| | + | |- |
| | + | |StyI (Eco130I) |
| | + | |1 uL |
| | + | |- |
| | + | |MilliQ |
| | + | |0 or 13 uL |
| | + | |- |
| | + | |Total |
| | + | |20 uL |
| | + | |- |
| | + | |} |
| | + | |
| | + | |} |
| | + | ** Expected fragments are |
| | + | ***pSB1A2-Lac: 1400, 650, 180, 40bp |
| | + | ***pSB3K3-const. promoter: 2620, 160bp |
| | + | ***pSB3K3: 3100bp |
| | + | ***pSB1AC3-const. promoter: 1770, 1319bp |
| | + | ***pSB1AC3: 3400bp |
| | + | **Loaded on a 1% Agarose gel (TBE) |
| | + | **Contents are (from left to rigth): |
| | + | 1KB marker, pSB2K3-H, pSB2K3-M, pSB2K3-L, pSB2K3, pSB1AC3-H, pSB1AC3-M, pSB1AC3-L, pSB1AC3, pSB1A2-Lac1, pSB1A2-Lac2. |
| | + | [[Image:F102471 2009-07-27 restriction2.JPG|250px]] |
| | + | **The fragments seem to be on the correct higth, though there are also extra bands seen, which may be uncut plasmid DNA. |
| | + | |
| | + | |
| | + | *'''O/n cultures of pBAD''' |
| | + | **Pick 4 colonies of TOP10 + pSB1A2-pBAD/AraC |
| | + | **Grow o/n in ~4ml LB-amp in the warm room. |
| | | | |
| | ==Dry== | | ==Dry== |
| | + | Finished [[Team:Groningen/Promoters|RPU computations]]! |
| | | | |
| | {{Team:Groningen/Notebook/Day/Footer}} | | {{Team:Groningen/Notebook/Day/Footer}} |
Latest revision as of 09:56, 31 July 2009
Wet
GVP Cluster
Transporters
GlpF
PCR 2 was add on gel again as noted below at accumulation.
PCR 1 was
GlpF PCR1
PCR1
| Component | amount
|
| 2x Phusion MM
| 12.5 uL
|
| MQ
| 10.25 uL
|
| GlpF Fw
| 1 uL
|
| GlpF MutRev
| 1 uL
|
| DNA GlpF Full Lenght (extracted as noted below at accumulation)(not added to tube nr2)
| 0.25 uL
|
|
|
| GlpF PCR1 program
| Temperature
| Time
|
| Denaturing
| 98°
| 2.00 min
|
| Start Cycles 30X
|
| Denaturing
| 98°
| 10 sec
|
| Annealing
| 60°
| 10 sec
|
| Elongation
| 72°
| 10 sec
|
|
| End cycles
|
| Final elongation
| 72°
| 5 min
|
| Hold
| 4°
| Forever
|
|
|
|
A 2% agarose gel was used, a band of 105 bp was expected.
The band in row 2 was cut and purified using the zymo research Zymoclean TM Gel DNA Recovery Kit.
The purified PCR1 DNA and PCR2 DNA were used to run PCR3
GlpF PCR3
PCR1.1
| Component | amount
|
| 2x Phusion MM
| 12.5 uL
|
| GlpF PCR1
| 6 uL
|
| GlpF PCR2
| 6 uL
|
|
|
| GlpF PCR1 program
| Temperature
| Time
|
| Denaturing
| 98°
| 2.00 min
|
| Start Cycles 30X
|
| Denaturing
| 98°
| 10 sec
|
| Annealing
| 60°
| 10 sec
|
| Elongation
| 72°
| 30 sec
|
|
| End cycles
|
| Final elongation
| 72°
| 5 min
|
| Hold
| 4°
| Forever
|
|
|
|
The results of the 1% agarose gel can be found at 28/07/09
Metal Accumulation
Gel with ArsR PCR((24/07/09)
ArsR/GlpF gel extraction
- - Used zymo research Zymoclean TM Gel DNA Recovery Kit to extract ArsR, GlpF PCR2 and GlpF FullLenght from gel (cut from gel as shown in figure above).
GlpF PCR1
PCR Mix
| 12,5 uL
| Phusion
|
| 1 uL
| GlpF FW primer
|
| 1 uL
| GlpF RevMut primer
|
| 0,25 uL
| GlpF Full Lenght DNA (extracted as noted above)(not in tube2)
|
| 10,25 uL
| MQ
|
Vectors
- Restriction digest on pSB3K3-const. promoters and pSB1AC3-cons. promoters and pSB1A2-lac promoter
- Plasmids isolated with sigma plasmid isolation kit from o/n culture 24-25 July
- Restriction was done with 250-500ng plasmid
|
|
|
Check pLac insert
| 10x Tango buffer
| 2 uL
|
| pSB1A2-lac1&2 (19-22 ng/uL)
| 17 uL
|
| EaeI (CfrI)
| 1 uL
|
| Total
| 20 uL
|
|
|
Check const. promoter insert (without promoter single cut, with 2x
| 10x Orange buffer
| 2 uL
|
| pSB3K3-HML or pSB1AC3-HML
| 7 or 17 uL (depending on conc.)
|
| StyI (Eco130I)
| 1 uL
|
| MilliQ
| 0 or 13 uL
|
| Total
| 20 uL
|
|
- Expected fragments are
- pSB1A2-Lac: 1400, 650, 180, 40bp
- pSB3K3-const. promoter: 2620, 160bp
- pSB3K3: 3100bp
- pSB1AC3-const. promoter: 1770, 1319bp
- pSB1AC3: 3400bp
- Loaded on a 1% Agarose gel (TBE)
- Contents are (from left to rigth):
1KB marker, pSB2K3-H, pSB2K3-M, pSB2K3-L, pSB2K3, pSB1AC3-H, pSB1AC3-M, pSB1AC3-L, pSB1AC3, pSB1A2-Lac1, pSB1A2-Lac2.
- The fragments seem to be on the correct higth, though there are also extra bands seen, which may be uncut plasmid DNA.
- O/n cultures of pBAD
- Pick 4 colonies of TOP10 + pSB1A2-pBAD/AraC
- Grow o/n in ~4ml LB-amp in the warm room.
Dry
Finished RPU computations!
| April
|
| M | T | W | T | F | S | S
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| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
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| May
|
| M | T | W | T | F | S | S
|
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|
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_May_2009 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_May_2009 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/31_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 31]
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| June
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| M | T | W | T | F | S | S
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_June_2009 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
|
| [http://2009.igem.org/Team:Groningen/Notebook/8_June_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
|
| [http://2009.igem.org/Team:Groningen/Notebook/15_June_2009 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_June_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_June_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_June_2009 19]
| [http://2009.igem.org/Team:Groningen/Notebook/20_June_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_June_2009 21]
|
| [http://2009.igem.org/Team:Groningen/Notebook/22_June_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_June_2009 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_June_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_June_2009 25]
| [http://2009.igem.org/Team:Groningen/Notebook/26_June_2009 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
|
|
| July
|
| M | T | W | T | F | S | S
|
|
|
| [http://2009.igem.org/Team:Groningen/Notebook/1_July_2009 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_July_2009 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
|
| [http://2009.igem.org/Team:Groningen/Notebook/6_July_2009 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_July_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_July_2009 8]
| [http://2009.igem.org/Team:Groningen/Notebook/9_July_2009 9]
| [http://2009.igem.org/Team:Groningen/Notebook/10_July_2009 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
|
| [http://2009.igem.org/Team:Groningen/Notebook/13_July_2009 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_July_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_July_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_July_2009 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_July_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
|
| [http://2009.igem.org/Team:Groningen/Notebook/20_July_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_July_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_July_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_July_2009 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_July_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_July_2009 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
|
| [http://2009.igem.org/Team:Groningen/Notebook/27_July_2009 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_July_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_July_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_July_2009 30]
| [http://2009.igem.org/Team:Groningen/Notebook/31_July_2009 31]
|
|
| August
|
| M | T | W | T | F | S | S
|
|
|
|
|
|
| [http://2009.igem.org/Team:Groningen/Notebook/1_August_2009 1]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_August_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
|
| [http://2009.igem.org/Team:Groningen/Notebook/3_August_2009 3]
| [http://2009.igem.org/Team:Groningen/Notebook/4_August_2009 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_August_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_August_2009 6]
| [http://2009.igem.org/Team:Groningen/Notebook/7_August_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_August_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_August_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
|
| [http://2009.igem.org/Team:Groningen/Notebook/10_August_2009 10]
| [http://2009.igem.org/Team:Groningen/Notebook/11_August_2009 11]
| [http://2009.igem.org/Team:Groningen/Notebook/12_August_2009 12]
| [http://2009.igem.org/Team:Groningen/Notebook/13_August_2009 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_August_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_August_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_August_2009 16]
|
| [http://2009.igem.org/Team:Groningen/Notebook/17_August_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_August_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_August_2009 19]
| [http://2009.igem.org/Team:Groningen/Notebook/20_August_2009 20]
| [http://2009.igem.org/Team:Groningen/Notebook/21_August_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_August_2009 22]
| [http://2009.igem.org/Team:Groningen/Notebook/23_August_2009 23]
|
| [http://2009.igem.org/Team:Groningen/Notebook/24_August_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_August_2009 25]
| [http://2009.igem.org/Team:Groningen/Notebook/26_August_2009 26]
| [http://2009.igem.org/Team:Groningen/Notebook/27_August_2009 27]
| [http://2009.igem.org/Team:Groningen/Notebook/28_August_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_August_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_August_2009 30]
|
| [http://2009.igem.org/Team:Groningen/Notebook/31_August_2009 31]
|
|
| September
|
| M | T | W | T | F | S | S
|
|
| [http://2009.igem.org/Team:Groningen/Notebook/1_September_2009 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_September_2009 2]
| [http://2009.igem.org/Team:Groningen/Notebook/3_September_2009 3]
| [http://2009.igem.org/Team:Groningen/Notebook/4_September_2009 4]
| [http://2009.igem.org/Team:Groningen/Notebook/5_September_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_September_2009 6]
|
| [http://2009.igem.org/Team:Groningen/Notebook/7_September_2009 7]
| [http://2009.igem.org/Team:Groningen/Notebook/8_September_2009 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/Team:Groningen/Notebook/10_September_2009 10]
| [http://2009.igem.org/Team:Groningen/Notebook/11_September_2009 11]
| [http://2009.igem.org/Team:Groningen/Notebook/12_September_2009 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
|
| [http://2009.igem.org/Team:Groningen/Notebook/14_September_2009 14]
| [http://2009.igem.org/Team:Groningen/Notebook/15_September_2009 15]
| [http://2009.igem.org/Team:Groningen/Notebook/16_September_2009 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_September_2009 17]
| [http://2009.igem.org/Team:Groningen/Notebook/18_September_2009 18]
| [http://2009.igem.org/Team:Groningen/Notebook/19_September_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
|
| [http://2009.igem.org/Team:Groningen/Notebook/21_September_2009 21]
| [http://2009.igem.org/Team:Groningen/Notebook/22_September_2009 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/Team:Groningen/Notebook/24_September_2009 24]
| [http://2009.igem.org/Team:Groningen/Notebook/25_September_2009 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
|
| [http://2009.igem.org/Team:Groningen/Notebook/28_September_2009 28]
| [http://2009.igem.org/Team:Groningen/Notebook/29_September_2009 29]
| [http://2009.igem.org/Team:Groningen/Notebook/30_September_2009 30]
|
|
| October
|
| M | T | W | T | F | S | S
|
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
| [http://2009.igem.org/Team:Groningen/Notebook/2_October_2009 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
|
| [http://2009.igem.org/Team:Groningen/Notebook/5_October_2009 5]
| [http://2009.igem.org/Team:Groningen/Notebook/6_October_2009 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
|
| [http://2009.igem.org/Team:Groningen/Notebook/19_October_2009 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/31_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 31]
|
|
| November
|
| M | T | W | T | F | S | S
|
|
|
|
|
|
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 6]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
| [http://2009.igem.org/Team:Groningen/Notebook/14_November_2009 14]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 16]
| [http://2009.igem.org/Team:Groningen/Notebook/17_November_2009 17]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 21]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 23]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
|
| [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
|
|
"
