EPF-Lausanne/1 August 2009

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<font size="6" color="#007CBC"><i>31 July 2009</i></font>  
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People in the lab will live at the rythm of the bacteria this week end :)
 
==Wet Lab==
==Wet Lab==
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We transformed competent bacteria with our different ligation products from the previous day:
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We checked the plates from the transformation of the previous day: only 7 clones grew on the LacI-RBS (not gel extracted) plate. We did a colony PCR with each of the clones that grew to check which clones potentially contained the desired fragment. To check this, we then ran agarose gel with samples of each of the PCRs. The result: 3 of the clones gave a fragment of the expected length!!!
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- neg control with vector only (Chl.)
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- neg control without any DNA (Amp.)
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- LacI-RBS from the gel extraction (Amp.)
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- LacI-RBS not gel-extracted (Amp.)
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- LacI-RBS-death cassette (Chl.)
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The bacteria were left to grow overnight at 37°C on the LB-agar plates.
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What was left of these clones was then put into liquid culture and left to incubate at 37°C.
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At around 30 past midnight minipreps of these clones were done!
==People in the lab==
==People in the lab==
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Christian, Mélanie, Nath, Basile, Gab
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Nath, Basile, Gab
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Latest revision as of 19:56, 2 August 2009



Contents

1 August 2009




Wet Lab

We checked the plates from the transformation of the previous day: only 7 clones grew on the LacI-RBS (not gel extracted) plate. We did a colony PCR with each of the clones that grew to check which clones potentially contained the desired fragment. To check this, we then ran agarose gel with samples of each of the PCRs. The result: 3 of the clones gave a fragment of the expected length!!!

What was left of these clones was then put into liquid culture and left to incubate at 37°C.

At around 30 past midnight minipreps of these clones were done!

People in the lab

Nath, Basile, Gab



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