Team:Newcastle/Labwork/4 August 2009

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==Sporulation Tuning Team==
==Sporulation Tuning Team==
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Prof. Anne Moir from Sheffield University kindly sent us the spores of the genes cwlD and sleB/cwlJ
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* HC401 (FrpC2, cwlJ:: Kan, sleB-lacZ Ery R)
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* AM1877 (cwlD,Cm r, trp C2)
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In the protocol given to us by Prof. Moir, there are two methods (Methods A and B), which can be used to recover the cwlD spores. However, neither give complete restoration of germination.
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[Attach Prof. Moir's protocols]
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Referring to Prof. Moir's protocols, Method A is a fast method which results in partial germination of approximately 0.1%.
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Method B on the other hand, is a slow method which involves stripping of the spore coat layers for improved germination of approximately 10% recovery.
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Due to the lack of time, the group decided to go with Method A.
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Revision as of 16:57, 4 August 2009

Lab Session 04/08/2009

Sporulation Tuning Team

Prof. Anne Moir from Sheffield University kindly sent us the spores of the genes cwlD and sleB/cwlJ

  • HC401 (FrpC2, cwlJ:: Kan, sleB-lacZ Ery R)
  • AM1877 (cwlD,Cm r, trp C2)

In the protocol given to us by Prof. Moir, there are two methods (Methods A and B), which can be used to recover the cwlD spores. However, neither give complete restoration of germination.

[Attach Prof. Moir's protocols]

Referring to Prof. Moir's protocols, Method A is a fast method which results in partial germination of approximately 0.1%. Method B on the other hand, is a slow method which involves stripping of the spore coat layers for improved germination of approximately 10% recovery. Due to the lack of time, the group decided to go with Method A.




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