Virginia Commonwealth/6 June 2009
From 2009.igem.org
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*Mini-prep (plasmid purification) tutorial | *Mini-prep (plasmid purification) tutorial | ||
**Data from plasmid purification | **Data from plasmid purification | ||
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Latest revision as of 14:14, 5 August 2009
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Contents |
Saturday 06 June 2009
Results
- Note that undergraduates need key card access to the building.
- All samples from yesterday's cultivation grew well.
Tasks
- Harvested cells from cultivation
- Purified plasmids using the mini-prep kit
- Froze stock of cells carrying plasmids in box C-15
Wetlab
- Mini-prep (plasmid purification) tutorial
- Data from plasmid purification
Part No. | A260 (nm) | A280 (nm) | A260/A280 | Concentration (microgram/mililiter) | Amount (microgram) | Volume (microliter) | |
---|---|---|---|---|---|---|---|
J13002 | .026 | .018 | 1.44 | 52 | 1.3 | 25 | |
pSB1C3 | .085 | .050 | 1.700 | 170 | 9.69 | 57 | |
R0040 | .025 | .017 | 1.471 | 50 | 2.000 | 40 | |
E1010 | .063 | .038 | 1.658 | 126 | 4.536 | 36 | |
pSB4C5 | .101 | .059 | 1.712 | 202 | 7.676 | 38 | |
E0840 | .084 | .049 | 1.714 | 168 | 6.888 | 41 |
- Bussingkm 23:10, 6 June 2009 (UTC)
Note the Data recorded here was off by a factor of 10 for concentration and 1000 for amount and was edited on July 6th. MandM 15:28, 6 July 2009 (UTC)