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Team:Warsaw/Calendar-Main/29 July 2009
From 2009.igem.org
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| - | </ | + | <h3><div style="text-align: center;">Construction of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012 ">K177012</a> operon1_part2 </div></h3> |
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| + | <h4>Ania</h4> | ||
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| + | <p>Tasks:</p> | ||
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| + | <li>Digest the only only one promising clone obtained with SchI and PstI </li> | ||
| + | </ul> | ||
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Revision as of 14:29, 5 August 2009
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Cloning of the mgtc promoter into the pSB1A3 plasmid
Kamil
Tasks:
- Bacteria transformation
Methods:
- A 200μl batch o chemocompetent bacteria was transformed according to the protocol with 15μl of ligation mix.
- 1x and 10x (100μl each) concentrations of bacteria were incubated on Petri dishes containing LB broth and ampicilin.
- The bacteria were incubated overnight at 37°C.
Results:
- Both dishes yielded considerable amounts of white colonies (pSB1A3 has a white/red selection)
Conclusions:
- The ligation seems to be successful.
Construction of K177012 operon1_part2
Ania
Tasks:
- Digest the only only one promising clone obtained with SchI and PstI
