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Team:Warsaw/Calendar-Main/29 July 2009
From 2009.igem.org
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(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3><div style="text-align: center;">Cloning of the mgtc promoter into the pSB1A3 plasmid</div></h3> <h4>Kamil</h4> <br /> <p>Tasks:<...) |
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<p>Results:</p> | <p>Results:</p> | ||
<ul> | <ul> | ||
| - | <li>Both dishes yielded considerable amounts of white colonies ( | + | <li>Both dishes yielded considerable amounts of white colonies (pSB1A3 has a white/red selection)</li></ul> |
<br /> | <br /> | ||
<p>Conclusions:</p> | <p>Conclusions:</p> | ||
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</ul> | </ul> | ||
<br /> | <br /> | ||
| + | <h3><div style="text-align: center;">Construction of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012 ">K177012</a> operon1_part2 </div></h3> | ||
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| + | <h4>Ania</h4> | ||
| + | <br/> | ||
| + | <p>Tasks:</p> | ||
| + | <ul> | ||
| + | <li>Digest the only only one promising clone obtained with SchI and PstI to confirm the sequence</li> | ||
| + | </ul> | ||
| + | <p>Results:</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2009/e/e0/Jarek_Ania_290709.jpg"></img> | ||
| + | <p>Comments:</p> | ||
| + | The obtained fragments are of the wrong size. The clone is not correct. | ||
</html> | </html> | ||
Latest revision as of 14:33, 5 August 2009
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Cloning of the mgtc promoter into the pSB1A3 plasmid
Kamil
Tasks:
- Bacteria transformation
Methods:
- A 200μl batch o chemocompetent bacteria was transformed according to the protocol with 15μl of ligation mix.
- 1x and 10x (100μl each) concentrations of bacteria were incubated on Petri dishes containing LB broth and ampicilin.
- The bacteria were incubated overnight at 37°C.
Results:
- Both dishes yielded considerable amounts of white colonies (pSB1A3 has a white/red selection)
Conclusions:
- The ligation seems to be successful.
Construction of K177012 operon1_part2
Ania
Tasks:
- Digest the only only one promising clone obtained with SchI and PstI to confirm the sequence
Results:
Comments:
The obtained fragments are of the wrong size. The clone is not correct.
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