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Team:Warsaw/Calendar-Main/30 July 2009
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(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3><div style="text-align: center;">Cloning of the mgtc promoter into the pSB1A3 plasmid</div></h3> <h4>Kamil</h4> <br /> <p>Tasks:<...) |
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| + | <h3><div style="text-align: center;">Construction of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012 ">K177012</a> operon1_part2 </div></h3> | ||
| + | <h4>Ania</h4> | ||
| + | <br/> | ||
| + | <p>Tasks:</p> | ||
| + | <ul><li> | ||
| + | Repeating the cloning process again and labeling my samples before going on holidays. Honestly I do not remember now what we did. </li> | ||
| + | <li><p>Results:</p> | ||
| + | Not very promising so far | ||
| + | </li></ul> | ||
</html> | </html> | ||
Latest revision as of 14:40, 5 August 2009
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Cloning of the mgtc promoter into the pSB1A3 plasmid
Kamil
Tasks:
- Colonies transfer
- Plasmid isolation and digest
Methods:
- Selected white colonies were transfered to a new dish, liquid cultures were established along the way.
- The liquid cultures were incubated for 6h at 37°C with aeration.
- The plasmids were isolated according to the protocol (see at the end of the page).
- The plasmids were digested with EcoRI and PstI enzymes.
- The results were visualised with electrophoresis on 1% agarose gel.
NOTE: Steps 3 to 5 were not carried out by me and I cannot take credit for them.
Results:
- Gel Electrophoresis:
From left:
- GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
- Colonies from 1 to 10
- GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
NOTE: The upper band represents the plasmid backbone, the lower band is a bit odd. The mgtc promoter is roughly 500 bps in length while the only band except the backbone is somewhere around 1500 bps. Ligation picked up something completely wrong.
Conclusions:
- Back to the drawing board, again...
Construction of K177012 operon1_part2
Ania
Tasks:
- Repeating the cloning process again and labeling my samples before going on holidays. Honestly I do not remember now what we did.
Results:
Not very promising so far
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