Template:Team:KULeuven/5 August 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
(New page: Miniprep of pairs: * RBS + Sam5 {{kulpart|K238007}} conc: 101,3 ng/ul * RBS+Sam8 {{kulpart|I742106}} conc: 95.7 ng/ul * Sam8 {{kulpart|I742142}} conc: 81,8 ng/ul * ComT {{kulpart|I742107}}...)
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Restriction digestion
Restriction digestion
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* Sam8, EcoRI en SpeI
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A1: Sam8, EcoRI en SpeI <br>
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* Sam5, EcoRI en XbaI
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A2: Sam5, EcoRI en XbaI<br>
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+
<br>
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+
B1: RBS, EcoRI en SpeI<br>
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* RBS, EcoRI en SpeI
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B2: ComT, EcoRI en XbaI<br>
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* ComT, EcoRI en XbaI
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<br>
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C1: Fcs, EcoRI en SpeI <br>
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+
C2: Ech, EcoRI en XbaI<br>
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* Fcs, EcoRI en SpeI
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* Ech, EcoRI en XbaI
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The mixture was incubated in 37C for 1h30
The mixture was incubated in 37C for 1h30
Followed by gelelectroforesis of the digested parts
Followed by gelelectroforesis of the digested parts

Revision as of 14:16, 6 August 2009

Miniprep of pairs:

  • RBS + Sam5 conc: 101,3 ng/ul
  • RBS+Sam8 conc: 95.7 ng/ul
  • Sam8 conc: 81,8 ng/ul
  • ComT , conc: 122,9 ng/ul


Restriction digestion A1: Sam8, EcoRI en SpeI
A2: Sam5, EcoRI en XbaI

B1: RBS, EcoRI en SpeI
B2: ComT, EcoRI en XbaI

C1: Fcs, EcoRI en SpeI
C2: Ech, EcoRI en XbaI

The mixture was incubated in 37C for 1h30

Followed by gelelectroforesis of the digested parts

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