"
Template:Team:KULeuven/5 August 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
JochemDeen (Talk | contribs) |
JochemDeen (Talk | contribs) |
||
| Line 6: | Line 6: | ||
| - | Restriction digestion | + | Restriction digestion<br> |
A1: Sam8, EcoRI en SpeI <br> | A1: Sam8, EcoRI en SpeI <br> | ||
A2: Sam5, EcoRI en XbaI<br> | A2: Sam5, EcoRI en XbaI<br> | ||
Revision as of 14:17, 6 August 2009
Miniprep of pairs:
- RBS + Sam5 conc: 101,3 ng/ul
- RBS+Sam8 conc: 95.7 ng/ul
- Sam8 conc: 81,8 ng/ul
- ComT , conc: 122,9 ng/ul
Restriction digestion
A1: Sam8, EcoRI en SpeI
A2: Sam5, EcoRI en XbaI
B1: RBS, EcoRI en SpeI
B2: ComT, EcoRI en XbaI
C1: Fcs, EcoRI en SpeI
C2: Ech, EcoRI en XbaI
The mixture was incubated in 37C for 1h30
Followed by gelelectroforesis of the digested parts
