"
Template:Team:KULeuven/5 August 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
JochemDeen (Talk | contribs) |
JochemDeen (Talk | contribs) |
||
| Line 7: | Line 7: | ||
Restriction digestion<br> | Restriction digestion<br> | ||
| - | A1: Sam8, EcoRI en SpeI <br> | + | A1: '''Sam8''', EcoRI en SpeI <br> |
| - | A2: Sam5, EcoRI en XbaI<br> | + | A2: '''Sam5''', EcoRI en XbaI<br> |
<br> | <br> | ||
| - | B1: RBS, EcoRI en SpeI<br> | + | B1: '''RBS''', EcoRI en SpeI<br> |
| - | B2: ComT, EcoRI en XbaI<br> | + | B2: '''ComT''', EcoRI en XbaI<br> |
<br> | <br> | ||
| - | C1: Fcs, EcoRI en SpeI <br> | + | C1: '''Fcs''', EcoRI en SpeI <br> |
| - | C2: Ech, EcoRI en XbaI<br> | + | C2: '''Ech''', EcoRI en XbaI<br> |
The mixture was incubated in 37C for 1h30 | The mixture was incubated in 37C for 1h30 | ||
Followed by gelelectroforesis of the digested parts | Followed by gelelectroforesis of the digested parts | ||
Revision as of 14:26, 6 August 2009
Miniprep of pairs:
- RBS + Sam5 conc: 101,3 ng/ul
- RBS+Sam8 conc: 95.7 ng/ul
- Sam8 conc: 81,8 ng/ul
- ComT , conc: 122,9 ng/ul
Restriction digestion
A1: Sam8, EcoRI en SpeI
A2: Sam5, EcoRI en XbaI
B1: RBS, EcoRI en SpeI
B2: ComT, EcoRI en XbaI
C1: Fcs, EcoRI en SpeI
C2: Ech, EcoRI en XbaI
The mixture was incubated in 37C for 1h30
Followed by gelelectroforesis of the digested parts
