Team:Newcastle/Labwork/7 August 2009

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(Difference between revisions)
(Stochastic Switch Team)
(Stochastic Switch Team)
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B. subtilis 168 strain is already in the fridge. To test our biopbricks ww will use gfp-rrnb integration vector. It uses spectinamycin as the antibiotic,
B. subtilis 168 strain is already in the fridge. To test our biopbricks ww will use gfp-rrnb integration vector. It uses spectinamycin as the antibiotic,
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We prepared the spectinamycin solution as below:
+
We solved 65mg of the spectinamycin in 13 ml of water, purified the solution and placed it into the fridge.
  Prepared volume of the spectinamycin solution: 13 ml
  Prepared volume of the spectinamycin solution: 13 ml

Revision as of 15:20, 10 August 2009


Lab Session 05/08/09

Stochastic Switch Team

Today, we identified the strains and prepared the spectinamycin solution.

We will use the E.coli strain numbered as 2386.

B. subtilis 168 strain is already in the fridge. To test our biopbricks ww will use gfp-rrnb integration vector. It uses spectinamycin as the antibiotic,

We solved 65mg of the spectinamycin in 13 ml of water, purified the solution and placed it into the fridge.

Prepared volume of the spectinamycin solution: 13 ml
Concentration: 5mg/ml





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