Team:Newcastle/Labwork/10 August 2009

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Revision as of 15:37, 10 August 2009

Lab Session 10/08/09

Stochastic Switch Team

Today we pepared gfp-rrnb plasmids using midiprep. We followed GenElute's midiprep protocol.

We had two E.coli cultures inoculated with gfp-rrnb left overnight. Although they had teh sampels, we treated them individually.

We centrifuged the tubes at 16000rpm for 30 minutes before adding ethanol to rinse the DNAs. We then centriguged the tubes again for 10 minutes at 16000rpm.

We evaporated the tubes fro 5 minutes at the end.

Results

500px‎

Team Newcastle iGEM 2009 08 10 Sample 2.png



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