• Home
• Team
• Project
• Parts
• Modelling
• Sponsors
• Lab Book

Project

• Overview
• Choices Rationale
• Characterisation
• Safety
• Judging Comments

Sub-projects

• Metal Intake/Efflux
• Metal Sensing
• Sporulation Tuning
• Population Dynamics
• Stochastic Switch
• Metal Sequester
• Chassis
• Promoter Library

Wet Lab

• Lab Book
• Protocols

Meetings

• Meetings Calendar

Planning

• Timeline
• Ideas

Links

• Helping other teams
• Ethics
• T-Shirts
• iGEM Meetup
• Our City
• Fun and Games
• Sponsors
• Useful Links
• Contact Us
• Press Coverage

Contents 1 Lab Session 10/08/09 1.1 Stochastic Switch Team 1.1.1 Results 1.1.1.1 Sample 1 1.1.1.2 Sample 2 1.2 Chassis team

Lab Session 10/08/09

Stochastic Switch Team

Today we pepared gfp-rrnb plasmids using midiprep. We followed GenElute's midiprep protocol.

We had two E.coli cultures inoculated with gfp-rrnb left overnight. Although they had teh sampels, we treated them individually.

We centrifuged the tubes at 16000rpm for 30 minutes before adding ethanol to rinse the DNAs. We then centriguged the tubes again for 10 minutes at 16000rpm.

We evaporated the tubes fro 5 minutes at the end.

Results

We got good DNA concentrations for both samples. The results are below:

Sample 1

The concentration of DNA: 829.6 ng/uL
The ratio of DNA concentration to protein concentration(260/280): 1.88
The ratio of protein concentration to RNA concentration(260/230): 2.33

Sample 2

The concentration of DNA: 601.1 ng/uL
The ratio of DNA concentration to protein concentration(260/280): 1.90
The ratio of protein concentration to RNA concentration(260/230): 2.11

Chassis team

• Created germination solution without lysozyme.
• Borrowed Em from Colin Harwood's lab.
• Created a new MgCl Stock solution, as old one had bits of matter floating in it.
• Created KPh stock solution.
• Autoclaved solutions.
• Made 250ml LB and 250ml H20, autoclaved.
• Inoculated LB with Em and Kan, then poured plates.