Virginia Commonwealth/6 July 2009
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==Monday 06 July 2009== | ==Monday 06 July 2009== | ||
===Results=== | ===Results=== | ||
- | * | + | * n/a |
- | + | ||
---- | ---- | ||
+ | |||
===Tasks=== | ===Tasks=== | ||
- | '' | + | ''Maria and Afton'' |
*Gather information and choose parts for experiments (promoter, backbone, and RET) | *Gather information and choose parts for experiments (promoter, backbone, and RET) | ||
*Write running draft | *Write running draft | ||
*Transfer information to new lab notebook | *Transfer information to new lab notebook | ||
+ | [[User:Trentay|Trentay]] 19:36, 6 July 2009 (UTC) | ||
+ | |||
+ | |||
+ | ''Kevin and Adam'' | ||
+ | *Continue research and further analyze and hone the list of DNA sequences to be synthesized | ||
+ | |||
+ | |||
+ | ''Craig and Clay'' | ||
+ | * It was decided to continue work done by the 2008 Eidenburg team by engineering a metabolic pathway for limonene synthesis. Three parts are currently available in the distribution plates (BBa_I742111, BBa_K118024, and BBa_K118025). BBa_I742111 contains a ribosomal binding site attached to the limonene synthase gene (LIMS1) from citrus lemon. BBa_K118024 contains the former as well as ribosomal binding sites and a dxs gene and appY gene. BBa_K118025 contains the ribosomal binding sites and the three aforementioned genes as well as a promoter. We will begin by electrotransforming these parts into the NEB 10 beta strain of E. coli once the electrotransformation cuvettes arrive. | ||
---- | ---- | ||
====Wetlab==== | ====Wetlab==== | ||
- | '' | + | ''Maria and Afton'' |
*n/a | *n/a | ||
[[User:Trentay|Trentay]] 19:36, 6 July 2009 (UTC) | [[User:Trentay|Trentay]] 19:36, 6 July 2009 (UTC) |
Latest revision as of 15:45, 10 August 2009
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Contents |
Monday 06 July 2009
Results
- n/a
Tasks
Maria and Afton
- Gather information and choose parts for experiments (promoter, backbone, and RET)
- Write running draft
- Transfer information to new lab notebook
Trentay 19:36, 6 July 2009 (UTC)
Kevin and Adam
- Continue research and further analyze and hone the list of DNA sequences to be synthesized
Craig and Clay
- It was decided to continue work done by the 2008 Eidenburg team by engineering a metabolic pathway for limonene synthesis. Three parts are currently available in the distribution plates (BBa_I742111, BBa_K118024, and BBa_K118025). BBa_I742111 contains a ribosomal binding site attached to the limonene synthase gene (LIMS1) from citrus lemon. BBa_K118024 contains the former as well as ribosomal binding sites and a dxs gene and appY gene. BBa_K118025 contains the ribosomal binding sites and the three aforementioned genes as well as a promoter. We will begin by electrotransforming these parts into the NEB 10 beta strain of E. coli once the electrotransformation cuvettes arrive.
Wetlab
Maria and Afton
- n/a
Trentay 19:36, 6 July 2009 (UTC)