Team:Newcastle/Project/Labwork/OurProtocols/Spectrophotometer

From 2009.igem.org

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=Using spectrometer==
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* Use a sensitive pipet set to 2ul. After each load clean the spectrometer with a tissue.
* Use a sensitive pipet set to 2ul. After each load clean the spectrometer with a tissue.
 +
* Run the program called Nucleic acids and select Nucleic acid menu option.
* Wash the spectrometer with 2ul of water.
* Wash the spectrometer with 2ul of water.
 +
* Click OK in the program
 +
* If there is a warning message with options, select "Empty data viewer buffer" option.
* Get a blank measurement (Click to blank menu option)  
* Get a blank measurement (Click to blank menu option)  
* Wash the spectrometer with 2ul of water.
* Wash the spectrometer with 2ul of water.
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* Load 2ul of sample
* Load 2ul of sample
* Measure the concentration. (Click to measure menu option)
* Measure the concentration. (Click to measure menu option)
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* Load other samples and measure the concentartions
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* Take a screenshot of the screen.
 +
* Load other samples and measure the concentrations
* Clean with 2ul of water
* Clean with 2ul of water
* Get a blank measurement
* Get a blank measurement

Revision as of 15:56, 10 August 2009

Using spectrometer

  • Use a sensitive pipet set to 2ul. After each load clean the spectrometer with a tissue.
  • Run the program called Nucleic acids and select Nucleic acid menu option.
  • Wash the spectrometer with 2ul of water.
  • Click OK in the program
  • If there is a warning message with options, select "Empty data viewer buffer" option.
  • Get a blank measurement (Click to blank menu option)
  • Wash the spectrometer with 2ul of water.
  • Get a blank measurement
  • Load 2ul of sample
  • Measure the concentration. (Click to measure menu option)
  • Take a screenshot of the screen.
  • Load other samples and measure the concentrations
  • Clean with 2ul of water
  • Get a blank measurement



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