Team:Newcastle/Labwork/11 August 2009

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==Chassis team==
==Chassis team==
*Recreated LB inoculated plates
*Recreated LB inoculated plates
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==Stochastic Switch Team
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Today we run the gel for our gfp-rrnb integration vector. We had restriction digest using EcoRI and HindIII. This gave us a 500bp of DNA on the gel.
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===Preparation of the gel===
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*3.2 gr of agarise were solved in 400ml of 1xTAE buffer to make 0.8% agarose gel
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*The solution was microwaved for 5 minutes and left to cool down
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 +
===Restriction digest===
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*We prepared a final solution of 10ul for the restriction digest
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*We used
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**4.75ul H2O
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**1ul 10xBuffer(BufferE)
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**0.25ul BSA
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**0.5ul HindIII
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**0.5ul EcoRI
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**3ul DNA (gfp-rrnb)
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===Running the gel===
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===Results===
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Revision as of 10:49, 12 August 2009

Contents

Lab Session 11/08/09

Chassis team

  • Recreated LB inoculated plates

==Stochastic Switch Team Today we run the gel for our gfp-rrnb integration vector. We had restriction digest using EcoRI and HindIII. This gave us a 500bp of DNA on the gel.

Preparation of the gel

  • 3.2 gr of agarise were solved in 400ml of 1xTAE buffer to make 0.8% agarose gel
  • The solution was microwaved for 5 minutes and left to cool down

Restriction digest

  • We prepared a final solution of 10ul for the restriction digest
  • We used
    • 4.75ul H2O
    • 1ul 10xBuffer(BufferE)
    • 0.25ul BSA
    • 0.5ul HindIII
    • 0.5ul EcoRI
    • 3ul DNA (gfp-rrnb)

Running the gel

Results



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