Team:Newcastle/Labwork/12 August 2009

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(New page: {{:Team:Newcastle/CSS}} {{:Team:Newcastle/Header}} {{:Team:Newcastle/Left}} =Lab Session 11/08/09= ==<u>Stochastic Switch Team</u>== Today we are trying to transform ''Bacillus subtilis''...)
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*LB + CHL + ''B. subtilis'' + diluted plasmid DNA (Cells should transform with the DNA)
*LB + CHL + ''B. subtilis'' + diluted plasmid DNA (Cells should transform with the DNA)
 +
*2xcompetence medium and 2xstravation medium were prepared.
 +
*We used the 2nd and the 3rd tubes for the tests
 +
*Incubated the samplesfor three hours at 37C
 +
*Added the starvation medium and incubated for another 2 hours
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*For each of the two cultures, added 0.4ml of the final solution from the culture and 10ul of DNA into an eppendorf tube.
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*Placed the tubes in the shaking incubator for an hour
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*...
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Revision as of 09:26, 13 August 2009

Lab Session 11/08/09

Stochastic Switch Team

Today we are trying to transform Bacillus subtilis with gfp-rrnb integration vector. Metal sensor team kindly inoculated B. subtilis cells into flask tubes and placed them into the shaking incubator. We labelled them as 1,2,3 and control.

We prepared four plates

  • LB + Bsubtilis (+ Control, cells should grow on this plate)
  • LB + CHL + B. subtilis (- control, cells will not grow on this plate)
  • LB + CHL + B. subtilis + plasmid DNA(Cells should transform with the DNA)
  • LB + CHL + B. subtilis + diluted plasmid DNA (Cells should transform with the DNA)
  • 2xcompetence medium and 2xstravation medium were prepared.
  • We used the 2nd and the 3rd tubes for the tests
  • Incubated the samplesfor three hours at 37C
  • Added the starvation medium and incubated for another 2 hours
  • For each of the two cultures, added 0.4ml of the final solution from the culture and 10ul of DNA into an eppendorf tube.
  • Placed the tubes in the shaking incubator for an hour
  • ...



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