Team:Newcastle/Labwork/7 August 2009

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(Difference between revisions)
(→Lab Session 07/08/09)
(→Sporulation Tuning/ Chassis Team)
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Referring to Method A of the protocol, it states that there should be 200ug per ml of buffer solution present.
Referring to Method A of the protocol, it states that there should be 200ug per ml of buffer solution present.
 +
We need:
We need:
  200ug Lysozyme in 100ul DI Water
  200ug Lysozyme in 100ul DI Water
  2mg Lysozyme in 1ml DI Water
  2mg Lysozyme in 1ml DI Water
 +
We made 0.5g of Lysozyme in 10ml of DI Water, where:
We made 0.5g of Lysozyme in 10ml of DI Water, where:
Line 31: Line 33:
  50mg Lysozyme in 1ml DI Water
  50mg Lysozyme in 1ml DI Water
  50ug Lysozyme in 1ul DI Water
  50ug Lysozyme in 1ul DI Water
 +
 +
 +
Therefore, the amount of Lysozyme stock solution to add to a ml of buffer solution is:
 +
50ug (Stock Solution) / 2ug (Desired Molarity) = 25
 +
In order to obtain a solution with a final concentration of 2ug/ul, the stock solution needs to be diluted 25 times.
 +
Desired volume = 1ml
 +
100ul / 25 = 4ul.
 +
 +
4ul of Lysozyme stock solution is needed per ml of buffer solution.
 +
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Revision as of 12:04, 13 August 2009

Lab Session 07/08/09

Stochastic Switch Team

Today, we identified the strains and prepared the spectinamycin solution.

We will use the E.coli strain numbered as 2386.

B. subtilis 168 strain is already in the fridge. To test our biopbricks ww will use gfp-rrnb integration vector. It uses spectinamycin as the antibiotic,

We solved 65mg of the spectinamycin in 13 ml of water, purified the solution and placed it into the fridge. 

Prepared volume of the spectinamycin solution: 13 ml
Concentration: 5mg/ml


Sporulation Tuning/ Chassis Team

Today, we prepared Lysozyme stock solution in preparation for the recovery of our cwlD spores kindly sent to us by Anne Moir from Sheffield University.

Referring to Method A of the protocol, it states that there should be 200ug per ml of buffer solution present.


We need: 

200ug Lysozyme in 100ul DI Water
2mg Lysozyme in 1ml DI Water


We made 0.5g of Lysozyme in 10ml of DI Water, where: 

0.5g Lysozyme in 10ml DI Water
50mg Lysozyme in 1ml DI Water
50ug Lysozyme in 1ul DI Water


Therefore, the amount of Lysozyme stock solution to add to a ml of buffer solution is: 

50ug (Stock Solution) / 2ug (Desired Molarity) = 25
In order to obtain a solution with a final concentration of 2ug/ul, the stock solution needs to be diluted 25 times.
Desired volume = 1ml
100ul / 25 = 4ul.

4ul of Lysozyme stock solution is needed per ml of buffer solution.




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